ANNEX IIISPECIFICATIONS FOR THE ANALYSIS OF PARAMETERS

Each Member State must ensure that any laboratory at which samples are analysed has a system of analytical quality control that is subject from time to time to checking by a person who is not under the control of the laboratory and who is approved by the competent authority for that purpose.

1.PARAMETERS FOR WHICH METHODS OF ANALYSIS ARE SPECIFIED

F1The following principles for methods of microbiological parameters are given either for reference, whenever a CEN/ISO method is given, or for guidance, pending the possible future adoption by the Commission of further CEN/ISO international methods for those parameters. Member States may use alternative methods, providing the provisions of Article 7(5) are met.

Those measures on further CEN/ISO international methods, designed to amend non-essential elements of this Directive, inter alia, by supplementing it, shall be adopted in accordance with the regulatory procedure with scrutiny referred to in Article 12(3).

  • Coliform bacteria and Escherichia coli (E. coli) (ISO 9308-1)

  • Enterococci (ISO 7899-2)

  • Pseudomonas aeruginosa (prEN ISO 12780)

  • Enumeration of culturable microorganisms — Colony count 22 °C (prEN ISO 6222)

  • Enumeration of culturable microorganisms — Colony count 37 °C (prEN ISO 6222)

  • Clostridium perfringens (including spores)

Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar (Note 1) at 44 ± 1 °C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.

Note 1:

The composition of m-CP agar is:

  • Basal medium

    Tryptose

    30 g

    Yeast extract

    20 g

    Sucrose

    5 g

    L-cysteine hydrochloride

    1 g

    MgSO4 · 7H2O

    0,1 g

    Bromocresol purple

    40 mg

    Agar

    15 g

    Water

    1 000 ml

  • Dissolve the ingredients of the basal medium, adjust pH to 7,6 and autoclave at 121 °C for 15 minutes. Allow the medium to cool and add:

    D-cycloserine

    400 mg

    Polymyxine-B sulphate

    25 mg

    Indoxyl-β-D-glucoside

    to be dissolved in 8 ml sterile water before addition

    60 mg

    Filter — sterilised 0,5 % phenolphthalein diphosphate solution

    20 ml

    Filter — sterilised 4,5 % FeCl3 · 6H2O

    2 ml

2.PARAMETERS FOR WHICH PERFORMANCE CHARACTERISTICS ARE SPECIFIED

2.1.

For the following parameters, the specified performance characteristics are that the method of analysis used must, as a minimum, be capable of measuring concentrations equal to the parametric value with a trueness, precision and limit of detection specified. Whatever the sensitivity of the method of analysis used, the result must be expressed using at least the same number of decimals as for the parametric value considered in Annex I, Parts B and C.

Parameters

Trueness % of parametric value(Note 1)

Precision % of parametric value(Note 2)

Limit of detection % of parametric value(Note 3)

Conditions

Notes

Acrylamide

To be controlled by product specification

Aluminium

10

10

10

Ammonium

10

10

10

Antimony

25

25

25

Arsenic

10

10

10

Benzo(a)pyrene

25

25

25

Benzene

25

25

25

Boron

10

10

10

Bromate

25

25

25

Cadmium

10

10

10

Chloride

10

10

10

Chromium

10

10

10

Conductivity

10

10

10

Copper

10

10

10

Cyanide

10

10

10

Note 4

1,2-dichloroethane

25

25

10

Epichlorohydrin

To be controlled by product specification

Fluoride

10

10

10

Iron

10

10

10

Lead

10

10

10

Manganese

10

10

10

Mercury

20

10

20

Nickel

10

10

10

Nitrate

10

10

10

Nitrite

10

10

10

Oxidisability

25

25

10

Note 5

Pesticides

25

25

25

Note 6

Polycyclic aromatic hydrocarbons

25

25

25

Note 7

Selenium

10

10

10

Sodium

10

10

10

Sulphate

10

10

10

Tetrachloroethene

25

25

10

Note 8

Trichloroethene

25

25

10

Note 8

Trihalomethanes — Total

25

25

10

Note 7

Vinyl chloride

To be controlled by product specification

2.2.

For hydrogen ion concentration the specified performance characterisatics are that the method of analysis used must be capable of measuring concentrations equal to the parametric value with a trueness of 0,2 pH unit and a precision of 0,2 pH unit.

Note 145:

Trueness is the systematic error and is the difference between the mean value of the large number of repeated measurements and the true value.

Note 245:

Precision is the random error and is usually expressed as the standard deviation (within and between batch) of the spread of results about the mean. Acceptable precision is twice the relative standard deviation.

These terms are further defined in ISO 5725.

Note 3:

Limit of detection is either:

  • three times the relative within batch standard deviation of a natural sample containing a low concentration of the parameter,

    or

  • five times the relative within batch standard deviation of a blank sample.

Note 4:

The method should determine total cyanide in all forms.

Note 5:

Oxidation should be carried out for 10 minutes at 100 °C under acid conditions using permanganate.

Note 6:

The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned. The limit of detection may not be achievable for all pesticides at present, but Member States should strive to achieve this standard.

Note 7:

The performance characteristics apply to the individual substances specified at 25 % of the parametric value in Annex I.

Note 8:

The performance characteristics apply to the individual substances specified at 50 % of the parametric value in Annex I.

3.PARAMETERS FOR WHICH NO METHOD OF ANALYSIS IS SPECIFIED

  • Colour

  • Odour

  • Taste

  • Total organic carbon

  • Turbidity (Note 1)

Note 1:

For turbidity monitoring in treated surface water the specified performance characteristics are that the method of analysis used must, as a minimum, be capable of measuring concentrations equal to the parametric value with a trueness of 25 %, precision of 25 % and a 25 % limit of detection.