ANNEX I U.K. TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp. SEPEDONICUS (Spieckermann et Kotthoff) Davis et al. SCOPE OF THE TEST SCHEME

Appendix 6 Validated PCR protocol and reagents

3. 10x Tris Acetate EDTA (TAE) buffer, pH 8,0 U.K.

Tris buffer	48,4 g
Glacial acetic acid	11,42 ml
EDTA (disodium salt)	3,72 g
Distilled water	1,00 L

Dilute to 1x before use.

Also commercially available (e.g. Invitrogen or equivalent).