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Commission Directive 2006/56/ECShow full title

Commission Directive 2006/56/EC of 12 June 2006 amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot

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Changes over time for: Division 9.1.

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EU Directives are published on this site to aid cross referencing from UK legislation. Since IP completion day (31 December 2020 11.00 p.m.) no amendments have been applied to this version.

9.1.Nutritional and enzymatic identification testsU.K.

Determine the following phenotypic properties which are universally present or absent in C. m. subsp. sepedonicus, according to the methods of Lelliott and Stead (1987), Klement et al. (1990), Schaad (2001), Anonymous (1987).

All media should be incubated at 21 °C and examined after six days. If no growth has occurred, incubate for up to 20 days.

All tests must include a known C. m. subsp.sepedonicus control. Nutritional and physiological tests must be made using inocula from nutrient agar subcultures. Morphological comparisons must be made from nutrient dextrose agar cultures.

Tests Expected result
Oxidation/Fermentation (O/F) testInert or weakly oxidative
Oxidase activity
Growth at 37 °C
Urease activity
Aesculin hydrolysis+
Starch hydrolysis– or weak
Tolerance of 7 % NaCl
Indole production
Catalase activity+
H2S production
Citrate utilization
Gelatin liquefaction
Acid glycerol
Acid from lactose– or weak
Acid from rhamnose
Acid from salicin
Gram stain (Appendix 9)+

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