ANNEX I

E 620 GLUTAMIC ACID

SynonymsL-Glutamic acid, L-α-aminoglutaric acid
Definition
Chemical nameL-Glutamic acid, L-2-amino-pentanedioic acid
Einecs200-293-7
Chemical formulaC5H9NO4
Molecular weight147,13
AssayContent not less than 99,0 % and not more than 101,0 % on the anhydrous basis
DescriptionWhite crystals or crystalline powder
Identification
A.Positive test for glutamic acid by thin layer chromatography
B.Specific rotation [α]D20
Between 31,5o and 32,2o
(10 % solution (anhydrous basis) in 2N HCl, 200 mm tube)
C.pH of a saturated solution
Between 3,0 and 3,5
Purity
Loss on dryingNot more than 0,2 % (80 oC, 3h)
Sulphated ashNot more than 0,2 %
ChlorideNot more than 0,2 %
Pyrrolidone carboxylic acidNot more than 0,2 %
LeadNot more than 2 mg/kg