ANNEX I

E 623 CALCIUM DIGLUTAMATE

Synonyms

Calcium glutamate

Definition

Chemical name

Monocalcium di-L-glutamate

Einecs

242-905-5

Chemical formula

C10H16CaN2O8 · x H2O (x = 0, 1, 2 or 4)

Molecular weight

332,32 (anhydrous)

Assay

Content not less than 98,0 % and not more than 102,0 % on the anhydrous basis

Description

White, practically odourless crystals or crystalline powder

Identification

A.

Positive test for calcium

B.

Positive test for glutamic acid by thin-layer chromatog-raphy

C.

Specific rotation [α]D20

Between 27,4 and 29,2 (for calcium diglutamate with x = 4) (10 % solution (anhydrous basis) in 2N HCl, 200 mm tube)

Purity

Water

Not more than 19,0 % (for calcium diglutamate with x = 4) (Karl Fischer)

Chloride

Not more than 0,2 %

Pyrrolidone carboxylic acid

Not more than 0,2 %

Lead

Not more than 2 mg/kg