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ANNEX I

E 635 DISODIUM 5'-RIBONUCLEOTIDE

SynonymsSodium 5'-ribonucleotide
Definition
Chemical nameDisodium 5'-ribonucleotide is essentially a mixture of disodium inosine-5'-monophosphate and disodium guanosine-5'-monophosphate
Chemical formulaC10H11N4Na2O8P · nH2O and
C10H12N5Na2O8P· nH2O
AssayContent of both major components not less than 97,0 %, and of each component not less than 47,0 % and not more than 53 %, in every case on the anhydrous basis
DescriptionOdourless, white or nearly white crystals or powder
Identification
A.Positive test for ribose, and for organic phosphate and for sodium
B.pH of a 5 % solution
Between 7,0 and 8,5
Purity
WaterNot more than 26,0 % (Karl Fischer)
Other nucleotidesNot detectable by thin-layer chromatography
LeadNot more than 2 mg/kg