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ANNEX X AU.K.ANALYSIS BY GAS CHROMATOGRAPHY OF METHYL ESTERS OF FATTY ACIDS

2.REAGENTSU.K.

2.1.Carrier gasU.K.

Inert gas (nitrogen, helium, argon, hydrogen, etc.), thoroughly dried and with an oxygen content of less than 10 mg/kg.

Note 1.U.K.

Hydrogen, which is used as a carrier gas only with capillary columns, can double the speed of analysis but is hazardous. Safety devices are available.U.K.

2.2.Auxiliary gasesU.K.

2.2.1.Hydrogen (purity ≥ 99,9 %), feree from organic impurities.U.K.
2.2.2.Air or oxygen, free from organic impurities.U.K.

2.3.Reference standardU.K.

A mixture of methyl esters of pure fatty acids, or the methyl esters of a fat of known composition, preferably similar to that of the fatty matter to be analyzed.

Care shall be taken to prevent the oxidation of polyunsaturated fatty acids.