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[F1ANNEX IV U.K. DETERMINATION OF WAX CONTENT BY CAPILLARY COLUMN GAS CHROMATOGRAPHY

6. EXPRESSION OF RESULTS U.K.

Indicate the total of the contents of the various C 40 to C 46 waxes in mg/kg fat (ppm).

NB : The components to be quantified refer to the peaks with carbon pair numbers between esters C 40 and C 46 , using the example of the olive oil wax chromatogram shown in the figure below. If ester C 46 appears twice, it is recommended that to identify it the fraction of the waxes of an olive-residue oil should be analysed where the C 46 peak is easy to identify because it is in the clear majority. U.K.

The results should be expressed to one decimal place.

Figure Chromatogram of the waxes of an olive oil] (1) U.K.

(1)

[F1After elution of the sterol esters the chromatogram trace must not show any significant peaks (triglycerides).]