Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis (c. 2568)

ANNEX V U.K. DETERMINATION OF THE COMPOSITION AND CONTENT OF STEROLS BY CAPILLARY-COLUMN GAS CHROMATOGRAPHY

3. APPARATUS U.K.

3.1. 250 ml flask fitted with a reflux condenser having ground-glass joints. U.K.

3.2. 500 ml separating funnels. U.K.

3.3. 250 ml flasks. U.K.

3.4. Complete apparatus for analysis by thin-layer chromatography using 20 × 20 cm glass plates. U.K.

3.5. Ultraviolet lamp having a wavelength of 366 or 254 nm. U.K.

3.6. 100 μl and 500 μl microsyringes. U.K.

3.7. A cylindrical filter funnel with a G3 porous septum (porosity 15 to 40 μm) of diameter approximately 2 cm and a depth of some 5 cm, with an attachment suitable for filtration under vacuum and a 12/21 male ground glass joint. U.K.

3.8. 50 ml vacuum conical flask with a 12/21 ground-glass female joint which can be fitted to the filter funnel (3.7). U.K.

3.9. A 10 ml test tube with a tapering bottom and a sealing stopper. U.K.

3.10. Gas chromatograph suitable for use with a capillary column, provided with a splitting system consisting of: U.K.

3.10.1. a thermostatic chamber for columns capable of maintaining the desired temperature with an accuracy of ± 1 °C; U.K.

3.10.2. a temperature-adjustable vaporization unit with a persilanized glass vapourizing element; U.K.

3.10.3. a flame ionization detector and converter-amplifier; U.K.

3.10.4. an integrator-recorder suitable for use with the converter-amplifier (3.10.3) having a response time of not more than one second and a variable paper speed. U.K.

3.11. A glass or fused-silica capillary column of length 20 to 30 m, internal diameter 0,25 to 0,32 mm, entirely coated with SE-52 or SE-54 liquid or equivalent in a uniform thickness between 0,10 and 0,30 μm. U.K.

3.12. A 10 μl gas chromatography microsyringe with a hardened needle. U.K.