Commission Regulation (EEC) No 2568/91Show full title

4. REAGENTS U.K.

4.1. Potassium hydroxide, [X1 approximately 2 mol/L ethanolic solution.] Dissolve 130 g of potassium hydroxide [X1 (minimum concentration 85 %)] with cooling in 200 ml of distilled water and then make up to one litre with ethanol. Keep the solution in well-stoppered dark glass bottles. U.K.

Editorial Information

X1 Substituted by Corrigendum to Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis (Official Journal of the European Communities No L 248 of 5 September 1991).

4.2. [X1 Diethyl ether], analytical purity. U.K.

4.3. Anhydrous sodium sulphate, analytical purity. U.K.

4.4. Glass plates coated with silica gel, without fluorescence indicator, thickness 0,25 mm (commercially available ready for use). U.K.

4.5. Potassium hydroxide, [X1 0,2 mol/L] ethanolic solution. Dissolve 13 g of potassium hydroxide in 20 ml of distilled water and make up to one litre with ethanol. U.K.

4.6. Benzene, for chromatography. (See 5.2.2) U.K.

4.7. Acetone, for chromatography. (See 5.2.2) U.K.

4.8. Hexane, for chromatography. (See 5.2.2) U.K.

4.9. [X1 Diethyl ether], for chromatography. (See 5.2.2) U.K.

4.10. Chloroform, analytical purity. (See 5.2.2) U.K.

4.11. Reference solution for thin-layer chromatography: cholesterol or phytosterols, [F1 2 %] solution in chloroform. U.K.

Textual Amendments

F1 Substituted by Commission Regulation (EEC) No 183/93 of 29 January 1993 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis.

4.12. 2,7-dichlorofluorescein, 0,2 % ethanolic solution. Make slightly basic by adding a few drops of [X1 2 mol/L] alcoholic potassium hydroxide solution. U.K.

4.13. Anhydrous pyridine, for chromatography. U.K.

4.14. Hexamethyl disilazane. U.K.

4.15. Trimethylchlorosilane. U.K.

4.16. Reference solutions of sterol trimethylsilyl ethers. To be prepared at the time of use from pure sterols or mixtures of sterols obtained from oils containing them. U.K.

4.17. [X1 β-cholestanol], 0,2 % solution (m/V) in chloroform (internal standard). U.K.

4.18. Carrier gas: hydrogen or helium, gas-chromatographic purity. U.K.

4.19. Auxiliary gases: U.K.

• hydrogen, gas-chromatographic purity,

• air, gas-chromatographic purity.