F1ANNEX VDETERMINATION OF THE COMPOSITION AND CONTENT OF STEROLS AND TRITERPENES DIALCOHOLS BY CAPILLARY-COLUMN GAS CHROMATOGRAPHY

Annotations:

6.EXPRESSION OF THE RESULTS

6.1.Report individual sterol concentrations as mg/kg of fatty material and their sum as total sterols.

The composition of each of the individual sterols and of the erythrodiol and uvaol should be expressed to one decimal point.

Total sterol composition must be expressed without any decimal point.

F26.2.Calculate the percentage of each individual sterol from the ratio of the relevant peak area to the total peak area for sterols:

sterolx=AxA×100math

where:

Ax

peak area for x;

ΣA

total peak area for sterols.

6.3.

Apparent β-sitosterol: Δ5-23-stigmastadienol + clerosterol + β-sitosterol + sitostanol + Δ5-avenasterol + Δ5-24-stigmastadienol.

6.4.Calculate the percentage of erythrodiol and uvaol:

Erythrodiol+ Uvaol=Er+ UvEr+ Uv+ ΣA× 100math

where

ΣA

sum area for sterol in computing system counts;

Er

area of Erythrodiol in computing system counts;

Uv

area of Uvaol in computing system counts;