Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis (c. 2568)

[F1 ANNEX VII U.K. DETERMINATION OF THE PERCENTAGE OF 2-GLYCERYL MONOPALMITATE

Textual Amendments

F1 Substituted by Commission Regulation (EC) No 702/2007 of 21 June 2007 amending Commission Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis.

4. REAGENTS U.K.

4.1. Silica gel with a grain size of between 0,063 and 0,200 mm (70/280 mesh) prepared as follows: Place the silica gel in a porcelain capsule, dry in an incubator at 160 °C for four hours, then leave to cool at room temperature in a desiccator. Add water equivalent to 5 % of the mass of the silica gel as follows: Weigh 152 g silica gel into an Erlenmeyer flask then add 8 g of distilled water, stopper and shake gently to distribute the water evenly. Leave to stand for at least 12 hours before use. U.K.

4.2. n-hexane (for chromatography) U.K.

4.3. Isopropanol U.K.

4.4. Isopropanol, 1/1 (v/v) aqueous solution U.K.

4.5. Pancreatic lipase. It must have an activity of between 2,0 and 10 lipase units per mg. (Pancreatic lipases with an activity of between 2 and 10 units per mg enzyme are commercially available.) U.K.

4.6. Buffer solution of trishydroxymethylaminomethane: 1 M aqueous solution adjusted to pH 8 (potentiometric control) by conc. HCl (1/1 v/v) U.K.

4.7. Enzyme-quality sodium cholate, 0,1 % aqueous solution (this solution must be used within two weeks of its preparation) U.K.

4.8. Calcium chloride, 22 % aqueous solution U.K.

4.9. Diethyl ether for chromatography U.K.

4.10. Developer solvent: mixture of n-hexane/diethyl ether (87:13 v:v) U.K.

4.11. Sodium hydroxide, 12 % by weight solution U.K.

4.12. Phenolphthalein, 1 % solution in ethanol U.K.

4.13. Carrier gas: hydrogen or helium, for gas chromatography U.K.

4.14. Auxiliary gases: hydrogen, 99 % minimum purity, free from moisture and organic substances, and air, for gas chromatography, of the same purity U.K.

4.15. Silanisation reagent: mixture of pyridine/hexamethyldisilazane, trimethylchlorosilane 9/3/1 (v/v/v). (Ready-to-use solutions are commercially available. Other silylation reagents may be used, particularly bis-trimethylsilyl trifluoracetamide + 1 % trimethylchlorosilane, diluted with an identical volume of anhydrous pyridine.) U.K.

4.16. Reference samples: pure monoglycerides or monoglyceride mixtures with a known percentage composition similar to that of the sample.] U.K.