Commission Regulation (EEC) No 2568/91 of 11 July 1991 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis (c. 2568)

[F1 ANNEX XVII U.K. METHOD FOR THE DETERMINATION OF STIGMASTADIENES IN VEGETABLE OILS

Textual Amendments

F1 Inserted by Commission Regulation (EC) No 656/95 of 28 March 1995 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis and Council Regulation (EEC) No 2658/87 on the tariff and statistical nomenclature and on the Common Customs Tariff.

4. APPARATUS U.K.

4.1. 250 ml flasks suitable for use with a reflux condenser. U.K.

4.2. Separating funnels of 500 ml capacity. U.K.

4.3. 100 ml round-bottom flasks. U.K.

4.4. Rotary evaporator. U.K.

4.5. Glass chromatography column (1,5 to 2,0 cm internal diameter by 50 cm length) with Teflon tap and a plug of glass wool fibre or sintered glass disc at the bottom. To prepare silica gel column, pour hexane into the chromatography column to a depth of approximately 5 cm and then fill with a slurry of silica gel in hexane (15 g in 40 ml) with the help of hexane portions. Allow to settle and finish settling by applying slight vibration. Add anhydrous sodium sulphate to a height of approximately 0,5 cm, finally elute the excess hexane. U.K.

4.6. Gas chromatograph with flame ionization detector, split or cold on-column injector and oven programmable to within ± 1 °C. U.K.

4.7. Fused silica capillary column for gas chromatography (0,25 or 0,32 mm internal diameter by 25 m length) coated with 5 %-phenylmethylsilicone phase, 0,25 mm film thickness. U.K.

Note 1: U.K.

Other columns of similar or lower polarity can be used. U.K.

4.8. Integrator-recorder with possibility of valley-valley integration mode. U.K.

4.9. 5 to 10 ml microsyringe for gas chromatography with cemented needle. U.K.

4.10. Electrical heating mantle or hot place.] U.K.