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Methods for standardisation of materials and procedures for examination, sampling and testing for:
Avian influenza
Newcastle disease
Annex III to Council Directive 92/66/EEC(3); or
Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organisation for animal Health (OIE);
Where Article 12 of Directive 90/539/EEC applies, the sampling and testing methods must comply with the methods described in Annexes to Commission Decision 92/340/EEC(4).
Salmonella pullorum and Salmonella gallinarum
Chapter III of Annex II to Directive 90/539/EEC; or
Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organisation for animal Health (OIE).
Salmonella arizonae
Serological examination: 60 birds must be sampled at the point of lay with methods described in the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organisation for animal Health (OIE).
Mycoplasma gallisepticum
Chapter III of Annex II to Directive 90/539/EEC; or
Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organisation for animal Health (OIE).
Mycoplasma meleagridis
Chapter III of Annex II to Directive 90/539/EEC.
Salmonella of public health significance
The detection method recommended by the Community reference laboratory (CRL) for Salmonella in Bilthoven, the Netherlands, or an equivalent method shall be used. That method is described in the current version of draft Annex D of ISO 6579 (2002): ‘Detection of Salmonella spp. in animal faeces and in samples of the primary production stage’. In that detection method, a semi-solid medium (modified semi-solid Rappaport-Vassiladis medium, MSRV) is used as the single selective enrichment medium.
Serotyping shall be carried out following the Kauffmann-White scheme or an equivalent method.
During the period referred to in point II.1 of Annex VIII, the official veterinarian shall take samples from the imported poultry for virological examination, to be tested as follows:
Between the seventh and the fifteenth day following the date of commencement of the isolation period, cloacal swabs must be taken from all birds where the consignment contains less than 60 birds, and from at least 60 birds where consignments contain more than 60 birds;
Testing of samples must be carried out in official laboratories designated by the competent authority, using diagnostic procedures for:
avian influenza as laid down in the diagnostic manual in Commission Decision 2006/437/EC;
Newcastle disease as laid down in Annex III to Council Directive 92/66/EEC.
Samples may be pooled, subject to a maximum of five samples from individual birds in each pool.
Virus isolates must be sent without delay to the national reference laboratory.
http://www.oie.int/eng/normes/mmanual/A_summry.htm
OJ L 260, 5.9.1992, p. 1. Directive as last amended by Directive 2006/104/EC (OJ L 363, 20.12.2006, p. 352).