Commission Regulation (EC) No 761/2009Show full title

3. REPORTING U.K.

3.1. TEST REPORT U.K.

The test report must include the following:

Test substance:

• physical nature and physicochemical properties, including water solubility limit,

• chemical identification data (e.g. CAS Number), including purity.

Test species:

• scientific name, clone (if known) and source.

Test conditions:

• test procedure used (static, semi-static or flow-through),

• date of start of the test and its duration,

• test medium,

• description of the experimental design: test vessels and covers, solution volumes, number of colonies and fronds per test vessel at the beginning of the test,

• test concentrations (nominal and measured as appropriate) and number of replicates per concentration,

• methods of preparation of stock and test solutions including the use of any solvents or dispersants,

• temperature during the test,

• light source, light intensity and homogeneity,

• pH values of the test and control media,

• test substance concentrations and the method of analysis with appropriate quality assessment data (validation studies, standard deviations or confidence limits of analyses),

• methods for determination of frond number and other measurement variables, e.g. dry weight, fresh weight or frond area,

• all deviations from this Testing Method.

Results:

• raw data: number of fronds and other measurement variables in each test and control vessel at each observation and occasion of analysis,

• means and standard deviations for each measurement variable,

• growth curves for each concentration (recommended with log transformed measurement variable, see second paragraph in section 2.2.1),

• doubling time/growth rate in the control based on the frond number,

• calculated response variables for each treatment replicate, with mean values and coefficient of variation for replicates,

• graphical representation of the concentration/effect relationship,

• estimates of toxic endpoints for response variables e.g. EC₅₀, EC₁₀, EC₂₀, and associated confidence intervals. If calculated, LOEC and/or NOEC and the statistical methods used for their determination,

• if ANOVA has been used, the size of the effect which can be detected (e.g. the least significant difference),

• any stimulation of growth found in any treatment,

• any visual signs of phytotoxicity as well as observations of test solutions,

• discussion of the results, including any influence on the outcome of the test resulting from deviations from this Testing Method.