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Commission Regulation (EC) No 761/2009 of 23 July 2009 amending, for the purpose of its adaptation to technical progress, Regulation (EC) No 440/2008 laying down test methods pursuant to Regulation (EC) No 1907/2006 of the European Parliament and of the Council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (Text with EEA relevance)
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OECD TG 221 (2006) Lemna Sp. Growth Inhibition Test.
The use of Lemna studies for coloured substances is detailed in Section 13.5.3 of the EU Manual of Decisions dated July 2006, at http://ecb.jrc.ec.europa.eu/new-chemicals
Guidance on information requirements and chemical safety assessment — Chapter R.7b: Endpoint specific guidance; Table 7.8.3 Summary of difficult substance testing issues, available at:
http://guidance.echa.europa.eu/docs/guidance_document/information_requirements_en.htm?time=1234958685#A
ASTM International. (2003). Standard Guide for Conducting Static Toxicity Test With Lemna gibba G3. E 1415-91 (Reapproved 1998). pp. 733-742. In, Annual Book of ASTM Standards, Vol. 11.05 Biological Effects and Environmental Fate; Biotechnology; Pesticides, ASTM, West Conshohocken, PA.
USEPA — United States Environmental Protection Agency. (1996). OPPTS 850.4400 Aquatic Plant Toxicity Test Using Lemna spp., ‘Public draft’. EPA 712-C-96-156. 8pp.
AFNOR — Association Française de Normalisation. (1996). XP T 90-337: Détermination de l’inhibition de la croissance de Lemna minor. 10pp.
SSI — Swedish Standards Institute. (1995). Water quality — Determination of growth inhibition (7-d) Lemna minor, duckweed. SS 02 82 13. 15pp. (in Swedish).
Environment Canada. (1999). Biological Test Method: Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte, Lemna minor. EPS 1/RM/37-120 pp.
Environment Canada. (1993) Proposed Guidelines for Registration of Chemical Pesticides: Non-Target Plant Testing and Evaluation. Canadian Wildlife Service, Technical Report Series No. 145.
Sims I., Whitehouse P., and Lacey R. (1999). The OECD Lemna Growth Inhibition Test. Development and Ring-testing of draft OECD Test Guideline. R&D Technical Report EMA 003. WRc plc — Environment Agency.
OECD (2000). Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures. OECD Environmental Health and Safety Publications, Series on Testing and Assessment No 23.
ISO DIS 20079. Water Quality — Determination of the Toxic Effect of Water Constituents and Waste Water to Duckweed (Lemna minor) — Duckweed Growth Inhibition Test.
Walbridge C. T. (1977). A flow-through testing procedure with duckweed (Lemna minor L.). Environmental Research Laboratory — Duluth, Minnesota 55804. US EPA Report No. EPA-600/3-77 108. September 1977.
Lockhart W. L., Billeck B. N. and Baron C. L. (1989). Bioassays with a floating plant (Lemna minor) for effects of sprayed and dissolved glyphosate. Hydrobiologia, 118/119, 353-359.
Huebert, D.B. and Shay J.M. (1993). Considerations in the assessment of toxicity using duckweeds. Environmental Toxicology and Chemistry, 12, 481-483.
Christensen, E.R., Nyholm, N. (1984): Ecotoxicological Assays with Algae: Weibull Dose-Response Curves. Env. Sci. Technol. 19, 713-718.
Nyholm, N. Sørensen, P.S., Kusk, K.O. and Christensen, E.R. (1992): Statistical treatment of data from microbial toxicity tests. Environ. Toxicol. Chem. 11, 157-167.
Bruce R.D. and Versteeg D.J. (1992). A statistical procedure for modelling continuous toxicity data. Environmental Toxicology and Chemistry, 11, 1485-1494.
OECD. (2004). Guidance Document on Statistical Analysis of Ecotoxicity Data.
Norberg-King T.J. (1988). An interpolation estimate for chronic toxicity: The ICp approach. National Effluent Toxicity Assessment Center Technical Report 05-88. USEPA, Duluth, MN.
Dunnett, C.W. (1955). A multiple comparisons procedure for comparing several treatments with a control. J. Amer. Statist. Assoc., 50, 1096-1121.
Dunnett, C.W. (1964). New tables for multiple comparisons with a control. Biometrics, 20, 482-491.
Williams, D.A. (1971). A test for differences between treatment means when several dose levels are compared with a zero dose control. Biometrics, 27: 103-117.
Williams, D.A. (1972). The comparison of several dose levels with a zero dose control. Biometrics, 28: 510-531.
Brain P. and Cousens R. (1989). An equation to describe dose-responses where there is stimulation of growth at low doses. Weed Research, 29, 93-96.
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