ANNEX

Identification number of the additive

Name of the holder of authorisation

Additive

Composition, chemical formula, description, analytical method

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff with a moisture content of 12 %

Category of zootechnical additives. Functional group: digestibility enhancers

4a12

Roal Oy

6-phytase

EC 3.1.3.26

  • Additive composition:

    • Preparation of 6-phytase (EC 3.1.3.26) produced by Trichoderma reesei

    • (CBS 122001) with a minimum activity of:

      • 40 000 PPU3/g in solid form

      • 10 000 PPU/g in liquid form

  • Characterisation of the active substance:

    6-phytase (EC 3.1.3.26) produced by Trichoderma reesei (CBS 122001)

  • Analytical method:4

    Colorimetric method quantifying the activity of 6-phytase by measuring released inorganic phosphate from sodium phytate by analysing the colour formed by reduction of a phosphomolybdate complex.

Poultry for fattening and breeding other than turkeys for fattening

—

250 PPU

—

1.

In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2.

Maximum recommended dose per kilogram of complete feed for all authorised species: 1 000 PPU

3.

For use in feed containing more than 0,23 % phytin- bound phosphorus.

4.

For safety: breathing protection, glasses and gloves shall be used during handling.

21.4.2020

Poultry for laying

125 PPU

Pigs other than sows

250 PPU

1 PPU is the amount of enzyme which liberates 1 μmol of inorganic phosphate from sodium phytate per minute at pH = 5.0 and 37C °.

Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives