Commission Regulation (EU) No 277/2010Show full title

ANNEX

a 1 PPU is the amount of enzyme which liberates 1 μmol of inorganic phosphate from sodium phytate per minute at pH = 5.0 and 37C °.
b Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives
Identification number of the additive	Name of the holder of authorisation	Additive	Composition, chemical formula, description, analytical method	Species or category of animal	Maximum age	Minimum content	Maximum content	Other provisions	End of period of authorisation
						Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a12	Roal Oy	6-phytase EC 3.1.3.26	Additive composition: Preparation of 6-phytase (EC 3.1.3.26) produced by Trichoderma reesei (CBS 122001) with a minimum activity of: 40 000 PPUa/g in solid form 10 000 PPU/g in liquid form Characterisation of the active substance: 6-phytase (EC 3.1.3.26) produced by Trichoderma reesei (CBS 122001) Analytical method:b Colorimetric method quantifying the activity of 6-phytase by measuring released inorganic phosphate from sodium phytate by analysing the colour formed by reduction of a phosphomolybdate complex.	Poultry for fattening and breeding other than turkeys for fattening	—	250 PPU	—	1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting. 2. Maximum recommended dose per kilogram of complete feed for all authorised species: 1 000 PPU 3. For use in feed containing more than 0,23 % phytin- bound phosphorus. 4. For safety: breathing protection, glasses and gloves shall be used during handling.	21.4.2020
				Poultry for laying		125 PPU
				Pigs other than sows		250 PPU