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a 1 PROT is the amount of enzyme that releases 1 μmol of p-nitroaniline from 1mM substrate (Suc-Ala-Ala-Pro-Phe-pNA) per minute at pH 9,0 and temperature 37 °C. | |||||||||
b Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives | |||||||||
Identification number of the additive | Name of the holder of authorisation | Additive | Composition, chemical formula, description, analytical method | Species or category of animal | Maximum age | Minimum content | Maximum content | Other provisions | End of period of authorisation |
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Units of activity/kg of complete feedingstuff with a moisture content of 12 % | |||||||||
Category of zootechnical additives. Functional group: digestibility enhancers | |||||||||
4a13 | DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp.Z.o.o. | Serine protease EC 3.4.21.- | Additive composition:
Characterisation of the active substance:
Analytical methodb: Colorimetric method measuring yellow complex para-nitroaniline (pNA) released by the enzyme from ‘Suc-Ala-Ala-Pro-Phe-pNA’ at pH 9,0 and at 37 °C | Chickens for fattening | — | 15 000 PROT | — | 1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.2.For safety reasons: breathing protection, glasses and gloves shall be used during handling. | 13.1.2020 |