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ANNEX

a

1 PROT is the amount of enzyme that releases 1 μmol of p-nitroaniline from 1mM substrate (Suc-Ala-Ala-Pro-Phe-pNA) per minute at pH 9,0 and temperature 37 °C.

b

Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives

Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a13DSM Nutritional Products Ltd, represented by DSM Nutritional Products Sp.Z.o.o.

Serine protease

EC 3.4.21.-

Additive composition:

  • Preparation of serine protease (EC 3.4.21.-)

  • produced by Bacillus licheniformis (DSM 19670) having a minimum activity of 75 000 PROTa/g

Characterisation of the active substance:

  • Serine protease (EC 3.4.21.-)

  • produced by Bacillus licheniformis (DSM 19670)

Analytical methodb:

Colorimetric method measuring yellow complex para-nitroaniline (pNA) released by the enzyme from ‘Suc-Ala-Ala-Pro-Phe-pNA’ at pH 9,0 and at 37 °C

Chickens for fattening15 000 PROT

1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2.For safety reasons: breathing protection, glasses and gloves shall be used during handling.

13.1.2020