ANNEX

Identification number of the additive

Name of the holder of authorisation

Additive

Composition, chemical formula, description, analytical method

Species or category of animal

Maximum age

Minimum content

Maximum content

Other provisions

End of period of authorisation

Units of activity/kg of complete feedingstuff with a moisture content of 12 %

Category of zootechnical additives. Functional group: digestibility enhancers

4a8

Roal Oy

Endo-1,4-beta-xylanase EC 3.2.1.8

  • Additive composition

    Preparation of endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 114044) having a minimum activity of:

    • solid form: 4 × 106 BXU4 /g

    • liquid form: 4 × 105 BXU/g

  • Characterisation of the active substance

    endo-1,4-beta-xylanase produced by Trichoderma reesei (CBS 114044)

  • Analytical method5

    In the additive and the premixture: reducing sugar assay for endo-1,4-beta-xylanase by colorimetric reaction of dinitrosalicylic acid reagent on reducing sugar yield at pH 5,3 and 50 °C

    In the feedingstuffs: colorimetric method measuring water soluble dye released by the enzyme from azurine crosslinked wheat arabinoxylan substrate

Minor poultry species other than laying birds thereof

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8 000 BXU

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  1. 1.

    In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

  2. 2.

    For safety: breathing protection, glasses and gloves shall be used during handling.

24 November 2021

Laying hens and laying birds of minor poultry species

24 000 BXU

Pigs for fattening

24 000 BXU

1 BXU is the amount of enzyme which liberates 1 nmol reducing sugars as xylose from birch xylan per second at pH 5,3 and 50 °C.

Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx