2.MONITORING IN LAYING FLOCKSU.K.
2.1.Frequency and status of samplingU.K.
Laying flocks shall be sampled at the initiative of the food business operator and by the competent authority.
Sampling at the initiative of the food business operator shall take place at least every 15 weeks. The first sampling shall take place at the flock-age of 24 +/– 2 weeks.
Sampling by the competent authority shall take place at least:
(a)
in one flock per year per holding comprising at least 1 000 birds;
(b)
at the age of 24 +/– 2 weeks in laying flocks housed in buildings where the relevant Salmonella was detected in the preceding flock;
(c)
in ... any cases where the competent authority considers it appropriate, using the sampling protocol laid down in point 4(b) of Part D to Annex II to Regulation (EC) No 2160/2003;
(d)
in all other laying flocks on the holding in case Salmonella Enteritidis or Salmonella Typhimurium is detected in one laying flock on the holding;
(e)
in cases where the competent authority considers it appropriate.
A sampling carried out by the competent authority may replace one sampling at the initiative of the food business operator.
2.2.Sampling protocolU.K.
In order to maximise the sensitivity of the sampling, and to ensure the correct application of the sampling protocol, the competent authority or the food business operator shall ensure that samples are taken by trained persons.
[2.2.1. Sampling by the food business operator U.K.
(a)
In cage flocks, 2 × 150 grams of naturally pooled faeces that have accumulated on scrapers or belt cleaners shall be taken from all belts or scrapers in the house after running the manure removal system; however, in the case of cage houses without scrapers or belts 2 × 150 grams of mixed fresh faeces must be collected from 60 different places beneath the cages in the dropping pits.
In cage houses where a sufficient amount of faeces does not accumulate on scrapers or belt cleaners at the discharge end of belts, four or more moistened fabric swabs of at least 900 cm 2 per swab shall be used to swab as large a surface area as possible at the discharge end of all accessible belts after they have been run, ensuring each swab is coated on both sides with faecal material from the belts and scrapers or belt cleaners.
(b)
In barn or free-range houses, two pairs of boot swabs or socks shall be taken.
Boot swabs used must be sufficiently absorptive to soak up moisture. The surface of the boot swab must be moistened using appropriate diluents.
The samples must be taken while walking through the house using a route that produces representative samples for all parts of the house or the respective sector. This shall include littered and slatted areas provided that slats are safe to walk on, but not areas outside the house in the case of flocks with outdoor access. All separate pens within a house must be included in the sampling. On completion of the sampling in the chosen sector, boot swabs must be removed carefully so as not to dislodge adherent material.
In multi-tier barn or free range houses in which most of the faecal material is removed from the house by dropping belts, one pair of boot swabs shall be taken by walking around in littered areas and at least a second pair of moistened fabric swabs shall be taken from all accessible dropping belts, as in the second paragraph of point (a).
The two samples can be pooled together to form one sample for testing.]
2.2.2.Sampling by the competent authorityU.K.
At least one sample must be collected using the sampling protocol in addition to samples referred to under point 2.2.1. Further samples shall be taken in order to ensure representative sampling if required by the distribution or the size of the flock.
In the case of sampling referred to in point 2.1(b), (c), (d) and (e), the competent authority shall satisfy itself by conducting further checks, namely by laboratory tests and/or documentary checks as appropriate to ensure that the results of examinations for Salmonella in birds are not affected by the use of antimicrobials in the flocks.
Where the presence of Salmonella Enteritidis and Salmonella Typhimurium is not detected but antimicrobials or bacterial growth inhibitory effects are detected it shall be considered and accounted for as an infected laying flock for the purpose of the ... target.
The competent authority may decide to allow replacement of one faecal sample or one pair of boot swabs by a dust sample of 100 grams collected from multiple places throughout the house from surfaces with a visible presence of dust. As an alternative one or several moistened fabric swab(s) of at least 900 cm2 surface area in total may be used instead to gather dust from multiple surfaces throughout the house, ensuring that each swab is well coated with dust on both sides.
The competent authority may decide to increase the minimum number of samples in order to ensure representative sampling on a case-by-case evaluation of epidemiological parameters, namely the biosecurity conditions, the distribution or size of the flock or other relevant conditions.