ANNEXU.K.

a

1 PPU is the amount of enzyme which liberates 1 μmol of inorganic phosphate from sodium phytate per minute at pH =5,0 and 37 °C.

b

Details of the analytical methods are available at the following address of the Community Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx

Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
Units of activity /kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a12Roal Oy6-phytase EC 3.1.3.26
  • Additive composition:

    Preparation of 6-phytase (EC 3.1.3.26) produced by Trichoderma reesei (CBS 122001) with a minimum activity of:

    • 40 000 PPUa /g in solid form

    • 10 000 PPU /g in liquid form

  • Characterisation of the active substance:

    of 6-phytase (EC 3.1.3.26) produced by Trichoderma reesei (CBS 122001)

  • Analytical method b :

    Colorimetric method quantifying the activity of 6-phytase by measuring released inorganic phosphate from sodium phytate by analysing the colour formed by reduction of a phosphomolybdate complex.

Sows250 PPU

1.In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

26 September 2021