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Commission Regulation (EU) No 231/2012Show full title
Commission Regulation (EU) No 231/2012 of 9 March 2012 laying down specifications for food additives listed in Annexes II and III to Regulation (EC) No 1333/2008 of the European Parliament and of the Council (Text with EEA relevance)
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- 2012 No. 231
- ANNEX
- E 140 (i) CHLOROPHYLLS
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- Revised 02/07/20202.48 MB
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E 140 (i) CHLOROPHYLLSU.K.
| Synonyms | CI Natural Green 3; Magnesium Chlorophyll; Magnesium Phaeophytin | |
| Definition | Chlorophylls are obtained by solvent extraction of strains of edible plant material, grass, lucerne and nettle. During the subsequent removal of solvent, the naturally present coordinated magnesium may be wholly or partly removed from the chlorophylls to give the corresponding phaeophytins. The principal colouring matters are the phaeophytins and magnesium chlorophylls. The extracted product, from which the solvent has been removed, contains other pigments such as carotenoids as well as oils, fats and waxes derived from the source material. Only the following solvents may be used for the extraction: acetone, methyl ethyl ketone, dichloromethane, carbon dioxide, methanol, ethanol, propan-2-ol and hexane. | |
| Colour Index No | 75810 | |
| Einecs | Chlorophylls: 215-800-7, chlorophyll a: 207-536-6, Chlorophyll b: 208-272-4 | |
| Chemical name | The major colouring principles are: Phytyl (132R,17S,18S)-3-(8-ethyl-132-methoxycarbonyl-2,7,12,18-tetramethyl-13′-oxo-3-vinyl-131-132-17,18-tetrahydrocyclopenta [at]-porphyrin-17-yl)propionate, (Phaeophytin a), or as the magnesium complex (Chlorophyll a) Phytyl (132R,17S,18S)-3-(8-ethyl-7-formyl-132-methoxycarbonyl-2,12,18-trimethyl-13′-oxo-3-vinyl-131-132-17,18-tetrahydrocyclopenta[at]-porphyrin-17-yl)propionate, (Pheophytin b), or as the magnesium complex (Chlorophyll b) | |
| Chemical formula | Chlorophyll a (magnesium complex): C55H72MgN4O5 Chlorophyll a: C55H74N4O5 Chlorophyll b (magnesium complex): C55H70MgN4O6 Chlorophyll b: C55H72N4O6 | |
| Molecular weight | Chlorophyll a (magnesium complex): 893,51 Chlorophyll a: 871,22 Chlorophyll b (magnesium complex): 907,49 Chlorophyll b: 885,20 | |
| Assay | Content of total combined Chlorophylls and their magnesium complexes is not less than 10 % | |
| Description | Waxy solid ranging in colour from olive green to dark green depending on the content of coordinated magnesium | |
| Identification | ||
|---|---|---|
| Spectrometry | Maximum in chloroform at ca. 409 nm | |
| Purity | ||
| Solvent residues | Acetone | Not more than 50 mg/kg, singly or in combination |
| Methyl Ethyl ketone | ||
| Methanol | ||
| Ethanol | ||
| Propan-2-ol | ||
| Hexane | ||
| Dichloromethane: | Not more than 10 mg/kg | |
| Arsenic | Not more than 3 mg/kg | |
| Lead | Not more than 5 mg/kg | |
| Mercury | Not more than 1 mg/kg | |
| Cadmium | Not more than 1 mg/kg | |
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