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Commission Regulation (EU) No 231/2012Show full title

Commission Regulation (EU) No 231/2012 of 9 March 2012 laying down specifications for food additives listed in Annexes II and III to Regulation (EC) No 1333/2008 of the European Parliament and of the Council (Text with EEA relevance)

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Changes over time for: E 957 THAUMATIN

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E 957 THAUMATIN U.K.

Synonyms
Definition
Einecs258-822-2
Chemical nameThaumatin is obtained by aqueous extraction (pH 2,5 to 4) of the arils of the fruit of strains of Thaumatococcus daniellii (Benth) and consists essentially of the proteins thaumatin I and thaumatin II together with minor amounts of plant constituents derived from the source material
Chemical formulaPolypeptide of 207 amino acids
Molecular weight

Thaumatin I 22209

Thaumatin II 22293

AssayNot less than 15,1 % nitrogen on the dried basis equivalent to not less than 93 % proteins (N × 6,2)
DescriptionOdourless, cream-coloured powder. Approximately 2 000 to 3 000 times as sweet as sucrose
Identification
SolubilityVery soluble in water, insoluble in acetone
Purity
Loss on dryingNot more than 9 % (105 °C to constant weight)
CarbohydratesNot more than 3 % (expressed on dry weight basis)
Sulphated ashNot more than 2 % (expressed on dry weight basis)
AluminiumNot more than 100 mg/kg (expressed on dry weight basis)
ArsenicNot more than 3 mg/kg (expressed on dry weight basis)
LeadNot more than 3 mg/kg (expressed on dry weight basis)
Microbiological criteria
Total aerobic microbial countNot more than 1 000 colonies per gram
Escherichia coliAbsent in 1 g

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