Commission Implementing Regulation (EU) No 98/2012Show full title

Commission Implementing Regulation (EU) No 98/2012 of 7 February 2012 concerning the authorisation of 6-phytase (EC 3.1.3.26) produced by Pichia pastoris (DSM 23036) as a feed additive for chickens and turkeys for fattening, chickens reared for laying, turkeys reared for breeding, laying hens, other avian species for fattening and laying, weaned piglets, pigs for fattening and sows (holder of authorisation Huvepharma AD) (Text with EEA relevance)

ANNEX

a

1 OTU is the amount of enzyme that catalyses the release of 1 micromole of inorganic phosphate per minute from 5,1 mM sodium phytate in pH 5,5 citrate buffer at 37 °C, measured as the blue P-molybdate complex colour at 820 nm.

b

Details of the analytical methods are available at the following address of the Community Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx

Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a16Huvepharma AD

6-phytase

(EC 3.1.3.26)

  • Additive composition:

    Preparation 6-phytase (EC 3.1.3.26) produced by Pichia pastoris (DSM 23036) with a minimum activity of:

    • 4 000 OUTa/g in solid form

    • 8 000 OTU/g in liquid form

  • Characterisation of the active substance:

    6-phytase (EC 3.1.3.26) produced by Pichia pastoris (DSM 23036)

  • Analytical method b:

    Colorimetric method based on the quantification of the inorganic phosphate released by the enzyme from the sodium phytate

Chickens for fattening

Chickens reared for laying

Laying hens

Other avian species other than turkeys for fattening and turkeys reared for breeding

Sows

—125 OTU—

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.

2. Recommended maximum dose for all authorised species: 500 OTU/kg of complete feedingstuff.

3. For use in feed containing more than 0,23 % phytin-bound phosphorus.

4. For safety: breathing protection, glasses and gloves shall be used during handling.

28 February 2022

Turkeys for fattening

Turkeys reared for breeding

Pigs for fattening

Piglets (weaned)

250 OTU