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ANNEX

a

1 BU is the amount of enzyme which liberates 1 nanomole of reducing sugars (expressed as glucose equivalents) from barley beta-glucan substrate per second at 50 °C and pH 4,8.

b

Details of the analytical methods are available at the following address of the Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx

Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentOther provisionsEnd of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers
4a20ROAL Oy

Endo-1,3(4)-beta-glucanase

EC 3.2.1.6

  • Additive composition

    Preparation of endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 126896) having a minimum activity of:

    • solid form: endo-1,3(4)-beta-glucanase 200 000 BUa/g,

    • liquid form: endo-1,3(4)-beta-glucanase 400 000 BU/ml.

  • Characterisation of the active substance

    endo-1,3(4)-beta-glucanase produced by Trichoderma reesei (CBS 126896)

  • Analytical method b

    For the quantification of endo-1,3(4)-beta-glucanase activity: spectrophotometric (DNS) method, based on the quantification of released sugars produced by the action of endo-1,3(4)-beta-glucanase on barley beta-glucan at pH 4,8 and 50 °C.

Chickens for fattening—20 000 BU—

1.In the directions for use of the additive and premixture, indicate the storage conditions and stability to pelleting.

2.For use in (weaned) piglets up to approximately 35 kg.

3.For safety: breathing protection, glasses and gloves shall be used during handling.

15 April 2024
Piglets (weaned)10 000 BU