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Commission Implementing Regulation (EU) 2015/1833 of 12 October 2015 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis
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Annex XIX to Regulation (EEC) No 2568/91 is amended as follows:
the title is replaced by the following:
‘DETERMINATION OF ALIPHATIC AND TRITERPENIC ALCOHOLS CONTENT BY CAPILLARY GAS CHROMATOGRAPHY’;
point 1 is replaced by the following:
This Annex describes a method for the determination of aliphatic and triterpenic alcohols content in oils and fats.’;
point 4.11 is replaced by the following:
points 5.2.5 and 5.2.6 are replaced by the following:
Wash the silica gel in the flask three times with ethyl ether (approximately 10 ml each time) collecting the filtrate in the same flask attached to the funnel. Evaporate the filtrate to a volume of 4 to 5 ml, transfer the residual solution to the previously weighed 10 ml test tube (3.9), evaporate to dryness by mild heating in a gentle flow of nitrogen, make up again using a few drops of acetone, evaporate again to dryness, place in an oven at 105 °C for approximately 10 minutes and then allow to cool in a desiccator and weigh.
The residue inside the test tube is composed of the alcoholic fraction.’;
point 5.4.4 is replaced by the following:
The identification of individual peaks is effected according to the retention times and by comparison with the standard TMSE mixture, analysed under the same conditions.
Examples of chromatogram of the alcoholic fraction of a refined olive oil is shown in Figures 2 and 3 of the Appendix.’;
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