ANNEXU.K.

a

Details of the analytical methods are available at the following address of the European Union Reference Laboratory for Feed Additives: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports

Identification number of the additiveName of the holder of authorisationAdditiveComposition, chemical formula, description, analytical methodSpecies or category of animalMaximum ageMinimum contentMaximum contentMinimum contentMaximum contentOther provisionsEnd of period of authorisation
CFU/kg of complete feedingstuff with a moisture content of 12 %CFU/l of water for drinking
Category of zootechnical additives. Functional group: gut flora stabilisers.
4b1715Centro Sperimentale del Latte Lactobacillus acidophilus CECT 4529

Additive composition

Preparation of Lactobacillus acidophilus CECT 4529 containing a minimum of: 5 × 1010 CFU/g of additive (solid form).

Characterisation of the active substance

Viable cells of Lactobacillus acidophilus CECT 4529.

Analytical method a

Enumeration: spread plate method (EN 15787).

Identification: Pulsed Field Gel Electrophoresis (PFGE).

Laying hens1 × 1095 × 108

1. In the directions for use of the additive, premixture and compound feed, indicate the storage conditions and stability to pelleting and in drinking water.

2. For safety: breathing protection shall be used during handling.

3. For use of the additive in water for drinking the homogeneous dispersion of the additive shall be ensured.

3 March 2025