[F1ANNEX 8U.K.List of plants, plant products and other objects originating in a CD territory or Great Britain and the special requirements for their introduction into Great Britain from a CD territory or their movement within Great Britain

Textual Amendments

F1Annex 8 substituted (31.12.2020) by The Plant Health (Phytosanitary Conditions) (Amendment) (EU Exit) Regulations 2020 (S.I. 2020/1527), reg. 1(2), Sch. 8 (as amended by S.I. 2020/1631, regs. 1(2), 9(2)(a))

Modifications etc. (not altering text)

C1Annex 8: power to amend conferred (31.12.2020) by Regulation (EU) No. 2016/2031, Art. 41(3)-(3B) (as substituted by The Plant Health (Amendment etc.) (EU Exit) Regulations 2020 (S.I. 2020/1482), regs. 2(2)(b), 29(4)(c))

PART BU.K.List of plants, plant products or other objects originating in a CD territory or Great Britain that are subject to emergency measures and may only be introduced into Great Britain from a CD territory or moved within Great Britain if special requirements are met

In this Part, “ISPM 31” has the same meaning as in Part B of Annex 7.

	(1)Description of plants, plant products or other objects	(2)Special requirements
1.	Plants for planting, other than seeds, of Viburnum spp. L., Camellia spp. L. and Rhododendron spp. L., other than Rhododendron simsii Planch,	The plants must be accompanied by: (a)an official statement that the plants originate in an area in which Phytophthora ramorum Werres, De Cock & Man in ‘t Veld is known not to occur, (b)an official statement that since the beginning of the last complete cycle of vegetation no signs of Phytophthora ramorum Werres, De Cock & Man in ‘t Veld have been observed on the plants at the place of production during official inspections, including laboratory testing of any suspicious symptoms, carried out at least twice during the growing season at appropriate times when the plants were in active growth and with an intensity which took into account the particular production system of the plants, or (c)where signs of Phytophthora ramorum Werres, De Cock & Man in ‘t Veld have been found on the plants at the place of production, an official statement that appropriate procedures have been implemented for the purpose of eradicating that pest and the plants have been found free from the pest following those procedures, which consisted of at least: (i)destruction of the infected plants and all susceptible plants within a 2 m radius of the infected plants, including associated growing media and plant debris, (ii)in the case of plants listed in column (1) of this entry within a 10 m radius of the infected plants and any remaining plants from the infected lot: (aa)they have been retained at the place of production, (bb)additional official inspections have been carried out at least twice in the three months after the eradication measures have been taken when the plants are in active growth, (cc)no treatments that may suppress symptoms of the pest have been carried out in that three month period, and (dd)the plants have been found free from the pest on these official inspections, (iii)in the case of all other plants listed in column (1) of this entry at the place of production, the plants have been subjected to intensive official re-inspection and have been found free from the pest on those inspections, and (iv)appropriate phytosanitary measures have been taken on the growing surface within a 2 m radius of infected plants.
2.	Seeds of Solanum lycopersicum L. and Capsicum spp., intended for planting, other than plants for planting of Capsicum spp. varieties which are known to be resistant to Tomato brown rugose fruit virus	The seeds must be accompanied by an official statement: (a)that the mother plants of seeds have been produced in a production site where Tomato brown rugose fruit virus is known not to occur on the basis of official inspections carried out at the appropriate time to detect that pest, (b)that the seeds or their mother plants have undergone sampling and testing for Tomato brown rugose fruit virus by the competent authority, or have been subjected to sampling and testing by professional operators under official supervision of the competent authority, and have been found, according to those tests, to be free from that pest, and (c)in the case of any seeds which were in storage prior to 15th August 2020, that the seeds have been sampled and tested for Tomato brown rugose fruit virus by the competent authority and found in those tests to be free from that pest. For the purposes of point (b), the sampling and testing of the seeds must be carried out in accordance with the paragraphs below. The official sampling of seeds for testing must be carried out in accordance with the following sampling schemes referred to in the relevant table of ISPM31: —in the case of seed lots which include 3000 or fewer seeds, a hypergeometric sampling scheme that is able to identify with 95% reliability a level of presence of infected plants of 10% or above, —in the case of seed lots which include 30000 or fewer seeds, but more than 3000 seeds, a sampling scheme that is able to identify with 95% reliability a level of presence of infected plants of 1% or above, —in the case of seed lots which include more than 30000 seeds, a sampling scheme that is able to identify with 95% reliability a level of presence of infected plants of 0.1% or above. Sub samples must consist of no more than 1000 seeds for Polymerase Chain Reaction (PCR) methods. The testing of seeds must be carried out using one of the following methods and the method used must be included in the phytosanitary certificate under the heading “Additional declaration”: —real-time RT-PCR using the primers and probes described in the ISF protocol (2020), or —real-time RT-PCR using primers and probe of Menzel and Winter (Acta Horticulturae, in press).
3.	Plants for planting of Solanum lycopersicum L. and Capsicum spp., other than plants for planting of Capsicum spp. varieties which are known to be resistant to Tomato brown rugose fruit virus	The plants must be accompanied by an official statement: (a)that the plants are derived from seeds which have undergone sampling and testing for Tomato brown rugose fruit virus in the manner set out in column (2) of entry 2 which has shown them to be free from that pest, and (b)that the plants have been produced in a production site where Tomato brown rugose fruit virus is known not to occur on the basis of official inspections carried out at the appropriate time to detect that pest, and, where the plants have shown symptoms of Tomato brown rugose fruit virus, the plants have undergone official sampling and testing for Tomato brown rugose fruit virus and have been found, according to those tests, to be free from that pest. For the purposes of point (b)(ii), the sampling and testing of the seeds must be carried out in accordance with the paragraphs below. In the case of plants for planting, 200 leaves must be collected per site of production and cultivar. In case of symptomatic plants, sampling for testing must be performed on at least 3 symptomatic leaves. One of the following testing methods must be carried out for the detection of Tomato brown rugose fruit virus: —in the case of symptomatic material only, ELISA, —conventional RT-PCR using the primers of Alkowni et al. (2019), —conventional RT-PCR using the primers of Rodriguez-Mendoza et al. (2019), —real-time RT-PCR using the primers and probes described in the ISF protocol (2020), —real-time RT-PCR using primers and probe of Menzel and Winter (Acta Horticulturae, in press). In case of a positive result of the detection test, a second testing method, different from the one used for detection, must be carried out with one of the RT-PCR methods mentioned above, using the same sample to confirm the identification.]

(1)Description of plants, plant products or other objects

(2)Special requirements

Plants for planting, other than seeds, of Viburnum spp. L., Camellia spp. L. and Rhododendron spp. L., other than Rhododendron simsii Planch,

The plants must be accompanied by:

(a)an official statement that the plants originate in an area in which Phytophthora ramorum Werres, De Cock & Man in ‘t Veld is known not to occur,

(b)an official statement that since the beginning of the last complete cycle of vegetation no signs of Phytophthora ramorum Werres, De Cock & Man in ‘t Veld have been observed on the plants at the place of production during official inspections, including laboratory testing of any suspicious symptoms, carried out at least twice during the growing season at appropriate times when the plants were in active growth and with an intensity which took into account the particular production system of the plants, or

(c)where signs of Phytophthora ramorum Werres, De Cock & Man in ‘t Veld have been found on the plants at the place of production, an official statement that appropriate procedures have been implemented for the purpose of eradicating that pest and the plants have been found free from the pest following those procedures, which consisted of at least:

(i)destruction of the infected plants and all susceptible plants within a 2 m radius of the infected plants, including associated growing media and plant debris,

(ii)in the case of plants listed in column (1) of this entry within a 10 m radius of the infected plants and any remaining plants from the infected lot:

(aa)they have been retained at the place of production,

(bb)additional official inspections have been carried out at least twice in the three months after the eradication measures have been taken when the plants are in active growth,

(cc)no treatments that may suppress symptoms of the pest have been carried out in that three month period, and

(dd)the plants have been found free from the pest on these official inspections,

(iii)in the case of all other plants listed in column (1) of this entry at the place of production, the plants have been subjected to intensive official re-inspection and have been found free from the pest on those inspections, and

(iv)appropriate phytosanitary measures have been taken on the growing surface within a 2 m radius of infected plants.

Seeds of Solanum lycopersicum L. and Capsicum spp., intended for planting, other than plants for planting of Capsicum spp. varieties which are known to be resistant to Tomato brown rugose fruit virus

The seeds must be accompanied by an official statement:

(a)that the mother plants of seeds have been produced in a production site where Tomato brown rugose fruit virus is known not to occur on the basis of official inspections carried out at the appropriate time to detect that pest,

(b)that the seeds or their mother plants have undergone sampling and testing for Tomato brown rugose fruit virus by the competent authority, or have been subjected to sampling and testing by professional operators under official supervision of the competent authority, and have been found, according to those tests, to be free from that pest, and

(c)in the case of any seeds which were in storage prior to 15th August 2020, that the seeds have been sampled and tested for Tomato brown rugose fruit virus by the competent authority and found in those tests to be free from that pest.

For the purposes of point (b), the sampling and testing of the seeds must be carried out in accordance with the paragraphs below.

The official sampling of seeds for testing must be carried out in accordance with the following sampling schemes referred to in the relevant table of ISPM31:

—in the case of seed lots which include 3000 or fewer seeds, a hypergeometric sampling scheme that is able to identify with 95% reliability a level of presence of infected plants of 10% or above,

—in the case of seed lots which include 30000 or fewer seeds, but more than 3000 seeds, a sampling scheme that is able to identify with 95% reliability a level of presence of infected plants of 1% or above,

—in the case of seed lots which include more than 30000 seeds, a sampling scheme that is able to identify with 95% reliability a level of presence of infected plants of 0.1% or above.

Sub samples must consist of no more than 1000 seeds for Polymerase Chain Reaction (PCR) methods.

The testing of seeds must be carried out using one of the following methods and the method used must be included in the phytosanitary certificate under the heading “Additional declaration”:

—real-time RT-PCR using the primers and probes described in the ISF protocol (2020), or

—real-time RT-PCR using primers and probe of Menzel and Winter (Acta Horticulturae, in press).

Plants for planting of Solanum lycopersicum L. and Capsicum spp., other than plants for planting of Capsicum spp. varieties which are known to be resistant to Tomato brown rugose fruit virus

The plants must be accompanied by an official statement:

(a)that the plants are derived from seeds which have undergone sampling and testing for Tomato brown rugose fruit virus in the manner set out in column (2) of entry 2 which has shown them to be free from that pest, and

(b)that the plants have been produced in a production site where Tomato brown rugose fruit virus is known not to occur on the basis of official inspections carried out at the appropriate time to detect that pest, and, where the plants have shown symptoms of Tomato brown rugose fruit virus, the plants have undergone official sampling and testing for Tomato brown rugose fruit virus and have been found, according to those tests, to be free from that pest.

For the purposes of point (b)(ii), the sampling and testing of the seeds must be carried out in accordance with the paragraphs below.

In the case of plants for planting, 200 leaves must be collected per site of production and cultivar.

In case of symptomatic plants, sampling for testing must be performed on at least 3 symptomatic leaves.

One of the following testing methods must be carried out for the detection of Tomato brown rugose fruit virus:

—in the case of symptomatic material only, ELISA,

—conventional RT-PCR using the primers of Alkowni et al. (2019),

—conventional RT-PCR using the primers of Rodriguez-Mendoza et al. (2019),

—real-time RT-PCR using the primers and probes described in the ISF protocol (2020),

—real-time RT-PCR using primers and probe of Menzel and Winter (Acta Horticulturae, in press).

In case of a positive result of the detection test, a second testing method, different from the one used for detection, must be carried out with one of the RT-PCR methods mentioned above, using the same sample to confirm the identification.]