ANNEX
The Annex to Regulation (EC) No 333/2007 is amended as follows:
- (1)
in point C.3.1, Definitions, the definitions of ‘LOD’ and ‘LOQ’ are replaced by the following:
- “LOD”
Limit of detection, smallest measured content, from which it is possible to deduce the presence of the analyte with reasonable statistical certainty.
- “LOQ”
Limit of quantification, lowest content of the analyte which can be measured with reasonable statistical certainty.
- (2)
in point C.3.3.1, Performance criteria, point (b) is replaced by the following:
- (b)
Performance criteria for methods of analysis for 3-monochloropropane-1,2-diol (3-MCPD), 3-MCPD fatty acid esters and glycidyl fatty acid esters:
Performance criteria for methods of analysis for 3-MCPD in foods specified in point 4.1 of the Annex to Regulation (EC) No 1881/2006
Table 6aParameter
Criterion
Applicability
Foods specified in point 4.1 of the Annex to Regulation (EC) No 1881/2006
Specificity
Free from matrix or spectral interferences
Field blanks
Less than LOD
Repeatability (RSDr)
0,66 times RSDR as derived from (modified) Horwitz equation
Reproducibility (RSDR)
as derived from (modified) Horwitz equation
Recovery
75-110 %
Limit of Detection (LOD)
≤ 5 μg/kg (on dry matter basis)
Limit of Quantification (LOQ)
≤ 10 μg/kg (on dry matter basis)
Performance criteria for methods of analysis for 3-MCPD in foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006
Table 6bParameter
Criterion
Applicability
Foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006
Specificity
Free from matrix or spectral interferences
Field blanks
Less than LOD
Repeatability (RSDr)
0,66 times RSDR as derived from (modified) Horwitz equation
Reproducibility (RSDR)
as derived from (modified) Horwitz equation
Recovery
75-110 %
Limit of Detection (LOD)
≤ 7 μg/kg
Limit of Quantification (LOQ)
≤ 14 μg/kg
Performance criteria for methods of analysis for 3-MCPD fatty acid esters, expressed as 3-MCPD, in foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006
Table 6cParameter
Criterion
Applicability
Foods specified in point 4.3 of the Annex to Regulation (EC) No 1881/2006
Specificity
Free from matrix or spectral interferences
Repeatability (RSDr)
0,66 times RSDR as derived from (modified) Horwitz equation
Reproducibility (RSDR)
as derived from (modified) Horwitz equation
Recovery
70-125 %
Limit of Detection (LOD)
Three tenths of LOQ
Limit of Quantification (LOQ)
for foods specified in 4.3.1 and 4.3.2
≤ 100 μg/kg in oils and fats
Limit of Quantification (LOQ)
for foods specified in 4.3.3 and in 4.3.4 with a fat content < 40 %
≤ two fifths of the ML
Limit of Quantification (LOQ)
for foods specified in 4.3.4 with a fat content ≥ 40 %
≤ 15 μg/kg fat
Performance criteria for methods of analysis for glycidyl fatty acid esters, expressed as glycidol, in foods specified in point 4.2 of the Annex to Regulation (EC) No 1881/2006
Table 6dParameter
Criterion
Applicability
Foods specified in point 4.2 of the Annex to Regulation (EC) No 1881/2006
Specificity
Free from matrix or spectral interferences
Repeatability (RSDr)
0,66 times RSDR as derived from (modified) Horwitz equation
Reproducibility (RSDR)
as derived from (modified) Horwitz equation
Recovery
70-125 %
Limit of Detection (LOD)
Three tenths of LOQ
Limit of Quantification (LOQ)
for foods specified in 4.2.1 and 4.2.2
≤ 100 μg/kg in oils and fats
Limit of Quantification (LOQ)
for foods specified in 4.2.3 with a fat content < 65 % and in 4.2.4 with a fat content < 8 %
≤ two fifths of the ML
Limit of Quantification (LOQ)
for foods specified in 4.2.3 with a fat content ≥ 65 % and in 4.2.4 with a fat content ≥ 8 %
≤ 31 μg/kg fat
- (b)
- (3)
in point C.3.3.1, Performance criteria, point (d), ‘Notes to the performance criteria’ is replaced by the following:
- (d)
Performance criteria for methods of analysis for acrylamide:
Table 8Parameter
Criterion
Applicability
All foods
Specificity
Free from matrix or spectral interferences
Field blanks
Less than Limit of Detection (LOD)
Repeatability (RSDr)
0,66 times RSDR as derived from (modified) Horwitz equation
Reproducibility (RSDR)
as derived from (modified) Horwitz equation
Recovery
75-110 %
Limit of Detection (LOD)
Three tenths of LOQ
Limit of Quantification (LOQ)
For foods with benchmark levels < 125 µg/kg: ≤ two fifths of the benchmark level, however not required to be lower than 20 µg/kg
For foods with benchmark level ≥ 125 µg/kg: ≤ 50 µg/kg
- (d)
- (4)
in point C.3.3.1, Performance criteria, the following points (e) and (f) are added:
- (e)
Performance criteria for methods of analysis for perchlorate:
Table 9Parameter
Criterion
Applicability
All foods
Specificity
Free from matrix or spectral interferences
Repeatability (RSDr)
0,66 times RSDR as derived from (modified) Horwitz equation
Reproducibility (RSDR)
as derived from (modified) Horwitz equation
Recovery
70-110 %
Limit of Detection (LOD)
Three tenths of LOQ
Limit of Quantification (LOQ)
≤ two fifths of the ML
- (f)
Notes to the performance criteria:
The Horwitz equation7 (for concentrations 1,2 x 10-7 ≤ C ≤ 0,138) and the modified Horwitz equation8 (for concentrations C < 1,2 × 10-7) are generalised precision equations which are independent of analyte and matrix but solely dependent on concentration for most routine methods of analysis.
Modified Horwitz equation for concentrations C < 1,2 × 10-7:
RSDR = 22 %
where:
RSDR is the relative standard deviation calculated from results generated under reproducibility conditions
C is the concentration ratio (i.e. 1 = 100g/100g, 0,001 = 1 000 mg/kg). The modified Horwitz equation applies to concentrations C < 1,2 × 10-7.
Horwitz equation for concentrations 1,2 x 10-7 ≤ C ≤ 0,138:
RSDR = 2C(-0,15)
where:
RSDR is the relative standard deviation calculated from results generated under reproducibility conditions
C is the concentration ratio (i.e. 1 = 100g/100g, 0,001 = 1 000 mg/kg). The Horwitz equation applies to concentrations 1,2 x 10-7 ≤ C ≤ 0,138.
- (e)
- (5)
in point C.3.3.2., ‘Fitness-for-purpose’ approach, the words ‘Table 8’ are replaced by the words ‘Table 10’.