ANNEX
Identification number of the additive	Name of the holder of authorisation	Additive	Composition, chemical formula, description, analytical method	Species or category of animal	Maximum age	Minimum content	Maximum content	Other provisions	End of period of authorisation
						Unit of activity/kg of complete feedingstuff with a moisture content of 12 %			
Category of zootechnical additives. Functional group: digestibility enhancers									
4a31	Andrés Pintaluba S.A.	6-phytase (EC 3.1.3.26)	Additive composition Preparation of 6-phytase (EC 3.1.3.26) produced by Komagataella phaffii (CGMCC 12056) having a minimum activity of: Solid form: 20 000 U/g Liquid form: 20 000 U/ml	laying hens and other laying birds	-	300 U	-	1. In the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment shall be indicated. 2. For users of the additive and premixtures, feed business operators shall establish operational procedures and organisational measures to address potential risks resulting from its use. Where those risks cannot be eliminated or reduced to a minimum by such procedures and measures, the additive and premixtures shall be used with personal protective equipment, including breathing protection.	21.12.2030
			Characterisation of the active substance 6-phytase (EC 3.1.3.26) produced by fermentation with Komagataella phaffii CGMCC 12056						
			Analytical method For the quantification of phytase activity in the feed additive: colorimetric method based on the enzymatic reaction of phytase on the phytate – VDLUFA 27.1.4 For the quantification of phytase activity in premixtures: colorimetric method based on the enzymatic reaction of phytase on the phytate -VDLUFA 27.1.3 For the quantification of phytase activity in feed materials and compound feed: colorimetric method based on the enzymatic reaction of phytase on the phytate – EN ISO 30024						
One unit is the amount of enzyme which releases one micromole of inorganic phosphate from phytate per minute at pH 5,5 and 37 °C.									
Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports									