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2002/106/EC: Commission Decision of 1 February 2002 approving a Diagnostic Manual establishing diagnostic procedures, sampling methods and criteria for evaluation of the laboratory tests for the confirmation of classical swine fever (Text with EEA relevance) (notified under document number C(2002) 381)
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Article 1.(1) Member States shall ensure that the confirmation of classical...
Article 2.Annexes I and IV to Council Directive 80/217/EEC of 22...
CLASSICAL SWINE FEVER DIAGNOSTIC MANUAL
CHAPTER II Description of classical swine fever with emphasis on differential diagnosis
1. Classical swine fever is caused by an enveloped RNA virus...
2. Classical swine fever virus is relatively stable in moist excretions...
3. The main natural route of infection is oro-nasal by direct...
4. The incubation period in individual animals is about one week...
5. The clinical signs of classical swine fever are extremely variable...
6. Acute, chronic and prenatal forms of classical swine fever can...
1. Weaners and fattening pigs most often display the acute form...
2. Classical swine fever virus causes severe leukopenia and immunosuppression, which...
3. Pathological changes visible on post-mortem examination are most frequently observed...
4. In general the acute form of African swine fever leads...
5. Classical swine fever virus is shed in saliva, urine and...
CHAPTER IV Checking and sampling procedures
A. Guidelines and procedures for clinical examination and sampling on pigs...
1. Member States shall ensure that appropriate clinical examinations, sampling and...
2. When an official veterinarian visits a suspected holding to confirm...
3. When reference is made to this paragraph, the clinical examination...
5. If further clinical signs or lesions that may suggest classical...
6. If the suspicion of classical swine fever in the holding...
7. If, after the examination carried out in a suspected holding,...
B. Sampling procedures in a holding when pigs are killed following...
C. Sampling procedures when pigs are killed as a preventive measure...
D. Checking and sampling procedures before authorisation is given to move...
E. Checking and sampling procedures in a holding in relation to...
F. Sampling procedures in holdings in the protection zone before lifting...
G. Sampling procedures in holdings in the surveillance zone before lifting...
H. Serological monitoring and sampling procedures in areas where classical swine...
CHAPTER V General procedures and criteria for collection and transport of samples
CHAPTER VI Principles and use of virological tests and evaluation of their results
1. Virus isolation is based on the incubation of sample material...
2. The preferred samples for isolation of classical swine fever virus...
3. Virus isolation is best suited for the investigation of samples...
4. It is recommended to perform virus isolation also in case...
5. All classical swine fever virus isolates from all primary outbreaks,...
CHAPTER VII Principles and use of serological tests and evaluation of their results
B. Recommended serological tests
1. The virus neutralisation test (VNT) and the ELISA are the...
4. The pestiviruses strains to be used in the neutralisation tests...
5. Several ELISA techniques using specific monoclonal antibodies have been developed,...
6. Quality control on sensitivity and specificity of each batch of...
7. The sensitivity of the ELISA is regarded as lower than...
C. Interpretation of serological results and differential diagnosis with infections due...
1. Without prejudice to the provisions of Article 4(3)(a) or Article...
2. If the comparative tests show antibodies to ruminant pestiviruses and...
3. If the comparative tests show a virus neutralisation titre in...
4. Without prejudice to the provisions of Article 4(3) of Directive...
5. However, if the further checks and tests referred to in...
CHAPTER VIII Discriminatory tests in case of emergency vaccination
CHAPTER IX Minimum safety requirements for classical swine fever laboratories
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