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Commission Regulation (EU) No 252/2012 (repealed)Show full title

Commission Regulation (EU) No 252/2012 of 21 March 2012 laying down methods of sampling and analysis for the official control of levels of dioxins, dioxin-like PCBs and non-dioxin-like PCBs in certain foodstuffs and repealing Regulation (EC) No 1883/2006 (Text with EEA relevance) (repealed)

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  8. Signature

    1. ANNEX I

      Definitions and abbreviations

      1. I. DEFINITIONS

      2. II. ABBREVIATIONS USED

    2. ANNEX II

      Methods of sampling for offical control of levels of dioxins (PCDD/PCDF), dioxin-like PCBs and non-dioxin-like PCBs in certain foodstuffs

      1. I. SCOPE

      2. II. GENERAL PROVISIONS

        1. 1. Personnel

        2. 2. Material to be sampled

        3. 3. Precautions to be taken

        4. 4. Incremental samples

        5. 5. Preparation of the aggregate sample

        6. 6. Replicate samples

        7. 7. Packaging and transmission of samples

        8. 8. Sealing and labelling of samples

      3. III. SAMPLING PLAN

        1. 1. Division of lots into sublots

        2. 2. Number of incremental samples

        3. 3. Specific provisions for the sampling of lots containing whole fishes...

        4. 4. Sampling of lots of fish containing whole fishes of different...

        5. 5. Sampling at retail stage

      4. IV. COMPLIANCE OF THE LOT OR SUBLOT WITH THE SPECIFICATION

        1. 1. As regards non-dioxin-like PCBs

        2. 2. As regards dioxins (PCDD/PCDF) and dioxin-like PCBs

      5. V. EXCEEDANCE OF ACTION LEVELS

    3. ANNEX III

      Sample preparation and requirements for methods of analysis used in offical control of the levels of dioxins (PCDD/PCDF) and dioxin-like PCBS in certain foodstuffs

      1. 1. FIELD OF APPLICATION

      2. 2. CLASSIFICATION OF METHODS BY THEIR DEGREE OF QUANTIFICATION

      3. 3. BACKGROUND

      4. 4. QUALITY ASSURANCE REQUIREMENTS

      5. 5. REQUIREMENTS FOR LABORATORIES

      6. 6. BASIC REQUIREMENTS TO BE MET BY ANALYTICAL PROCEDURE FOR DIOXINS...

        1. 6.1. Low working range and limits of quantification

        2. 6.2. High selectivity (specificity)

        3. 6.3. High accuracy (trueness and precision, bioassay apparent recovery)

        4. 6.4. Validation in the range of level of interest and general...

        5. 6.5. Limit of quantification

        6. 6.6. Analytical criteria

        7. 6.7. Specific requirements for screening methods

      7. 7. SPECIFIC REQUIREMENTS FOR GC/HRMS METHODS TO BE COMPLIED WITH FOR...

        1. 7.1. General requirements

        2. 7.2. Control of recoveries

        3. 7.3. Removal of interfering substances

        4. 7.4. Calibration with standard curve

      8. 8. SPECIFIC REQUIREMENTS FOR BIOANALYTICAL METHODS

        1. 8.1. Evaluation of the test response

          1. 8.1.1. General requirements

          2. 8.1.2. Calibration

            1. 8.1.2.1. Calibration with standard curve

            2. 8.1.2.2. Calibration with reference samples

          3. 8.1.3. Separate determination of PCDD/Fs and dioxin-like PCBs

          4. 8.1.4. Bioassay apparent recoveries

          5. 8.1.5. Control of recoveries for clean-up

          6. 8.1.6. Reporting limit

        2. 8.2. Use of reference samples

        3. 8.3. Determination of cut-off values

          1. 8.3.1. . . . . . . . . . ....

          2. 8.3.2. . . . . . . . . . ....

          3. 8.3.3. . . . . . . . . . ....

          4. 8.3.4. Restrictions to cut-off values:

        4. 8.4. Performance characteristics

      9. 9. REPORTING OF THE RESULT

        1. Confirmatory methods

        2. Bioanalytical screening methods

      10. Appendix to ANNEX III

        1. . . . . . . . . . ....

    4. ANNEX IV

      Sample preparation and requirements for methods of analysis used in offical control of the levels of non-dioxin-like PCBs (PCB # 28, 52, 101, 138, 153, 180) in certain foodstuffs

      1. 1. Applicable detection methods

      2. 2. Identification and confirmation of analytes of interest

      3. 3. Demonstration of performance of method

      4. 4. Limit of quantification

      5. 5. Quality control

      6. 6. Control of recoveries

      7. 7. Requirements for laboratories

      8. 8. Performance characteristics: Criteria for the sum of the six indicator...

      9. 9. Reporting of results

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