1 Use the following method to make m-CP agar: | |
| Parameter | Method |
|---|---|
| Clostridium perfringens (including spores) | Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar1 at 44 ±1ºC for 21± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds. |
| Coliform bacteria | BS-EN ISO 9308-1 |
| Colony count 22ºC – enumeration of culturable microorganisms | BS-EN ISO 6222 |
| Colony count 37ºC – enumeration of culturable microorganisms | BS-EN ISO 6222 |
| Enterococci | BS-EN ISO 7899-2 |
| Escherichia coli (E. Coli) | BS-EN ISO 9308-1 |
| Pseudomonas aeruginosa | BS-EN ISO 12780 |
Make a basal medium consisting of—
| Tryptose | 30.0g |
| Yeast extract | 20.0g |
| Sucrose | 5.0g |
| L-cysteine hydrochloride | 1.0g |
| MgSO4.7H2O | 0.1g |
| Bromocresol purple | 40.0mg |
| Agar | 15.0g |
| Water | 1,000.0ml |
| Dissolve the ingredients of basal medium, adjust pH to 7.6 and autoclave at 121ºC for 15 minutes. Allow the medium to cool. | |
| Dissolve— | |
| D-cycloserine | 400.0mg |
| Polymyxine-B sulphate | 25.0mg |
| Indoxl-β-D-glucoside | 60.0mg |
| into 8ml sterile water and add it to the medium. | |
| Add to the medium— | |
| Filter-sterilised 0.5% phenolphthalein diphosphate solution | 20.0ml |
| Filter-sterilised 4.5% FeCl3.6H2O | 2.0ml |
1 The method of analysis should determine total cyanide in all forms. | |||
2 Oxidation should be carried out for 10 minutes at 100ºC under acid conditions using permanganate. | |||
3 The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned. | |||
4 The performance characteristics apply to the individual substances specified at 25% of the parametric value in Table B in Part 2, Schedule 7. | |||
5 The performance characteristics apply to the individual substance specified at 50% of the parametric value in Table B in Part 2, Schedule 7. | |||
| Parameters | Trueness % of prescribed concentration or value specification | Precision % of prescribed concentration or value or specification | Limit of detection % of prescribed concentration or value or specification |
|---|---|---|---|
| Aluminium | 10 | 10 | 10 |
| Ammonium | 10 | 10 | 10 |
| Antimony | 25 | 25 | 25 |
| Arsenic | 10 | 10 | 10 |
| Benzene | 25 | 25 | 25 |
| Benzo(a)pyrene | 25 | 25 | 25 |
| Boron | 10 | 10 | 10 |
| Bromate | 25 | 25 | 25 |
| Cadmium | 10 | 10 | 10 |
| Chloride | 10 | 10 | 10 |
| Chromium | 10 | 10 | 10 |
| Conductivity | 10 | 10 | 10 |
| Copper | 10 | 10 | 10 |
| Cyanide1 | 10 | 10 | 10 |
| 1,2-dichloroethane | 25 | 25 | 10 |
| Fluoride | 10 | 10 | 10 |
| Iron | 10 | 10 | 10 |
| Lead | 10 | 10 | 10 |
| Manganese | 10 | 10 | 10 |
| Mercury | 20 | 10 | 20 |
| Nickel | 10 | 10 | 10 |
| Nitrate | 10 | 10 | 10 |
| Nititre | 10 | 10 | 10 |
| Oxidisability2 | 25 | 25 | 10 |
| Pesticides and related products3 | 25 | 25 | 25 |
| Polycyclic aromatic hydrocarbons4 | 25 | 25 | 25 |
| Selenium | 10 | 10 | 10 |
| Sodium | 10 | 10 | 10 |
| Sulphate | 10 | 10 | 10 |
| Tetrachloroethene5 | 25 | 25 | 10 |
| Tetrachloromethane | 20 | 20 | 20 |
| Trichloroethene5 | 25 | 25 | 10 |
| Trihalomethanes4 | 25 | 25 | 10 |