Regulations 2(1), 2(4) and 15(5)

SCHEDULE 3ANALYSIS: METHODS AND CAPABILITIES

TABLE M1

SPECIFIED PARAMETERS: ANALYTICAL METHOD TO BE USED

(1)

Item

(2)

Parameter

(3)

Method

(a)

The composition of m-CP agar is:

Basal medium—

Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes.

Tryptose30.0 g
Yeast extract20.0 g
Sucrose5.0 g
L-cysteine hydrochloride1.0 g
MgSO4.7H2O0.1 g
Bromocresol purple40.0 mg
Agar15.0 g
Water1,000.0 ml

Allow the medium to cool and add—

D-cycloserine400.0 mg
Polymyxine-B sulphate25.0 mg
Indoxyl-(beta)-D-glucoside to be dissolved in 8ml sterile water before addition60.0 mg
Filter-sterilised 0.5% phenolphthalein disphosphate solution20.0 ml
Filter-sterilised 4.5% FeCl3.6H2O2.0 ml
1.Clostridium perfringens (including spores)Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar(a) at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
2.Coliform bacteriaISO 9308-1
3.Colony count 22°C — enumeration of culturable microorganismsprEN ISO 6222
4.Colony count 37°C — enumeration of culturable microorganismsprEN 6222
5.EnterococciISO 7899-2
6.Escherichia coliISO 9308-1
Notes—

TABLE M2

OTHER PARAMETERS: CAPABILITIES REQUIRED OF ANALYTICAL METHOD

(1) Item(2) Parameter(3) Trueness % of prescribed concentration or value(4) Precision % of prescribed concentration or value(5) Limit of detection % of prescribed concentration or value
Notes—
(a)

The method of analysis should determine total cyanide in all forms.

(b)

The capabilities required apply in relation to each individual pesticide.

(c)

The capabilities required apply in relation to each individual substance comprising PAH: Total at 25% of the prescribed concentration or value for PAH: Total.

(d)

The capabilities required apply at 50% of the prescribed concentration or value for PAH: Total.

(e)

The capabilities required apply in relation to prescribed concentration or value in Table B.

(f)

The capabilities required apply in relation to prescribed concentration or value in Table C.

In this Schedule “Pesticide”, “PAH: Total” and “THM: Total” have the meanings given in Schedule 1.
1.Aluminium101010
2.Ammonium101010
3.Antimony252525
4.Arsenic101010
5.Benzene252525
6.Benzo(a)pyrene252525
7.Boron101010
8.Bromate252525
9.Cadmium101010
10.Chloride101010
11.Chromium101010
12.Colour101010
13.Conductivity101010
14.Copper101010
15.Cyanide(a)101010
16.1,2-dichloroethane252510
17.Fluoride101010
18.Iron101010
19.Lead101010
20.Manganese101010
21.Mercury201020
22.Nickel101010
23.Nitrate101010
24.Nitrite101010
25.Pesticide(b)252525
26.PAH: Total(c)252525
27.Selenium101010
28.Sodium101010
29.Sulphate101010
30.Tetrachloroethene(d)252510
31.Tetrachloromethane202020
32.Trichloroethene(d)252510
33.THM: Total(c)252510
34.Turbidity(e)101010
35.Turbidity(f)252525