SCHEDULE 2Testing Methods

PART 1METHOD FOR THE ISOLATION OF CLOSTRIDIUM PERFRINGENS

Inoculations3

For each portion of the sample 1 ml of solution shall be transferred to a sterile 90 mm petri dish (in duplicate), to which 15 ml of Shahidi – Ferguson agar (SF agar) M1 at a temperature of 47°C±1°C shall be added and immediately gently mixed by swirling the dish with 5 clockwise and 5 anticlockwise circular movements.