F1SCHEDULE 3METHODS OF ANALYSIS

Annotations:

PART AMicrobiological parameters

1

1

Subject to sub-paragraph (2), the methods in paragraph 2 are given for reference.

2

Scottish Water may use other methods, providing the provisions of regulation 14A are met.

2

The methods for microbiological parameters are—

a

for Escherichia coli and coliform bacteria—

i

European standard EN ISO 9308-1:2014 entitled “Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014)”; or

ii

European standard EN ISO 9308-2:2014 entitled “Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 2: Most probable number method (ISO 9308-2:2012)”;

b

for enterococci, European standard EN ISO 7899-2:2000 entitled “Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method (ISO 7899-2:2000)”;

c

for Pseudomonas aeruginosa, European standard EN ISO 16266:2008 entitled “Water quality - Detection and enumeration of Pseudomonas aeruginosa - Method by membrane filtration (ISO 16266:2006)”;

d

for colony count 22 °C (the enumeration of culturable microorganisms — colony count after aerobic incubation at 22 °C), European standard EN ISO 6222:1999 entitled “Water quality - Enumeration of culturable micro-organisms - Colony count by inoculation in a nutrient agar culture medium (ISO 6222:1999)”;

e

for colony count 36 °C (the enumeration of culturable microorganisms — colony count after aerobic incubation at 36 °C), European standard EN ISO 6222:1999 entitled “Water quality - Enumeration of culturable micro-organisms - Colony count by inoculation in a nutrient agar culture medium (ISO 6222:1999)”; and

f

for Clostridium perfringens including spores, European standard EN ISO 14189:2016 entitled “Water quality - Enumeration of Clostridium perfringens - Method using membrane filtration (ISO 14189:2013)”.