[F1SCHEDULE 3SMETHODS OF ANALYSIS

PART ASMicrobiological parameters

1.(1) Subject to sub-paragraph (2), the methods in paragraph 2 are given for reference.S

(2) Scottish Water may use other methods, providing the provisions of regulation 14A are met.

2.  The methods for microbiological parameters are—S

(a)for Escherichia coli and coliform bacteria—

(i)European standard EN ISO 9308-1:2014 entitled “Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014)”; or

(ii)European standard EN ISO 9308-2:2014 entitled “Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 2: Most probable number method (ISO 9308-2:2012)”;

(b)for enterococci, European standard EN ISO 7899-2:2000 entitled “Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method (ISO 7899-2:2000)”;

(c)for Pseudomonas aeruginosa, European standard EN ISO 16266:2008 entitled “Water quality - Detection and enumeration of Pseudomonas aeruginosa - Method by membrane filtration (ISO 16266:2006)”;

(d)for colony count 22 °C (the enumeration of culturable microorganisms — colony count after aerobic incubation at 22 °C), European standard EN ISO 6222:1999 entitled “Water quality - Enumeration of culturable micro-organisms - Colony count by inoculation in a nutrient agar culture medium (ISO 6222:1999)”;

(e)for colony count 36 °C (the enumeration of culturable microorganisms — colony count after aerobic incubation at 36 °C), European standard EN ISO 6222:1999 entitled “Water quality - Enumeration of culturable micro-organisms - Colony count by inoculation in a nutrient agar culture medium (ISO 6222:1999)”; and

(f)for Clostridium perfringens including spores, European standard EN ISO 14189:2016 entitled “Water quality - Enumeration of Clostridium perfringens - Method using membrane filtration (ISO 14189:2013)”.]