F1SCHEDULE 3METHODS OF ANALYSIS

Annotations:

PART AMicrobiological parameters

1

1

Subject to sub-paragraph (2), the methods in paragraph 2 are given for reference.

2

Scottish Water may use other methods, providing the provisions of regulation 14A are met.

2

The methods for microbiological parameters are—

a

for Escherichia coli and coliform bacteria—

i

European standard EN ISO 9308-1:2014 entitled “Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014)”; or

ii

European standard EN ISO 9308-2:2014 entitled “Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 2: Most probable number method (ISO 9308-2:2012)”;

b

for enterococci, European standard EN ISO 7899-2:2000 entitled “Water quality - Detection and enumeration of intestinal enterococci - Part 2: Membrane filtration method (ISO 7899-2:2000)”;

F2c

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d

for colony count 22 °C (the enumeration of culturable microorganisms — colony count after aerobic incubation at 22 °C), European standard EN ISO 6222:1999 entitled “Water quality - Enumeration of culturable micro-organisms - Colony count by inoculation in a nutrient agar culture medium (ISO 6222:1999)”;

F3e

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f

for Clostridium perfringens including spores, European standard EN ISO 14189:2016 entitled “Water quality - Enumeration of Clostridium perfringens - Method using membrane filtration (ISO 14189:2013)

F4g

for somatic coliphages, European Standard EN ISO 10705-2:2000 entitled “Water quality — Detection and enumeration of bacteriophages — Part 2: Enumeration of somatic coliphages” and European Standard EN ISO 10705-3:2003 entitled “Water quality — Detection and enumeration of bacteriophages — Part 3: Validation of methods for concentration of bacteriophages from water” can be used.