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The Processed Animal Protein Order 1989
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The incubated composite media or equivalent shall be examined and the findings recorded, discarding cultures which are obviously not salmonella. Slide seriological tests shall be performed using salmonella polyvalent “O” and polyvalent “H” (phase 1 and 2) agglutinating sera on selected suspect colonies collected from the blood agar or MacConkey plates. If reactions occur with one or both sera, the colonies shall be typed by slide serology and a subculture sent (in Scotland) to the Lasswade Veterinary Laboratory situated at Penicuik, Midlothian, and (in England and Wales) to a Veterinary Investigation Centre of the Ministry for further typing.
(a)Buffered Peptone Water/Lysine/Glucose (BPW/L/G)—Ogden (1988)
(b)Selenite Cystine TMAO Dulcitol (SC/T/D)—Eastern and Gibson (1985)
(c)Lysine Decarboxylase Glucose (LD/G)—Ogden (1988)
(d)Brilliant Green Agar (Modified) (BGA)—Edel and Kampelmacher (1969)
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