Regulation 10
1. The reference methods for the analysis and testing of dairy products in order to ascertain if they meet the requirements specified in Schedules 3, 4, 5 and 6 shall be those specified in the following paragraphs of this Schedule.
2. In relation to raw milk and heat-treated milk, the methods shall be those specified in Commission Decision 91/180/EEC(1) for the following:—
(a)enumeration of micro-organisms — plate count at 30°C;
(b)enumeration of somatic cells in cows' milk;
(c)determination of phosphatase activity in cows' milk;
(d)determination of peroxidase activity in cows' milk;
(e)detection of pathogenic micro-organisms;
(f)enumeration of coliforms — colony count at 30°C;
(g)enumeration of micro-organisms — plate count at 21°C.
3. In relation to milk-based products, the test for enumeration of micro-organisms — plate count at 30°C shall be the method specified in IDF Standard 100B: 1991; Milk and milk products — micro-organisms — colony count at 30°C.
4. In relation to milk-based products, the test for enumeration of coliforms shall be the method specified in 1SO 5541 Part 2 1986. Milk and milk products — Part 2; Most probable number technique at 30°C.
5. In relation to the following tests, the methods for the analysis and testing shall be those specified below:—
| Test | Method |
|---|---|
(a)Detection of Staphylococcus aureus | Provisional IDF Standard 145: 1990. Milk and milk-based products. Enumeration of Staphylococcus aureus in products other than dried milk-Colony count technique at 37°C. |
(b)Enumeration of Staphylococcus aureus in powdered milk | Provisional IDF Standard 138: 1986. Dried Milk. Enumeration of Staphylococcus aureus. Colony count technique at 37°C. |
(c)Detection of Salmonella spp | ISO 6579: 1993. Microbiology — General guidance on methods for the detection of Salmonella and IDF Standard 93A:1985; sample preparation. |
(d)Detection of Listeria monocytogenes | ISO 10560: 1993. Milk and milk products—Detection of Listeria monocytogenes. |
(e)Enumeration of Escherichia coli in milk-based products | Provisional IDF Standard 170: 1994. Milk and milk products. Enumeration of presumptive Escherichia Coli content by— (i) most probable number technique (ii) most probable number technique with use of 4—methylumbelliferyl—β—D—glucuronide (MUG) (iii) Colony count technique at 44°C using membranes. |
(f)Detection of phosphatase (Residual) in cream | AOAC official method 950.41: Official Methods of Analysis, 16th edition: 1995. |
(g)Detection of phosphatase (Reactivated and Residual) in cream | AOAC official method 965.27: Official Methods of Analysis, 16th edition: 1995. |
6. Any other internationally accepted analysis and test method as referred to in Article 20(1) of Council Directive 92/46 shall be recognised as a reference method.
7. In this Schedule—
“IDF Standard” means a standard of the International Dairy Federation;
“ISO” means a standard of the International Organisation for Standardisation; and
“AOAC” means the Association of Official Analytical Chemists.
OJ No. L93, 13.4.91, p.1.