The Animal By-Products Order 1999 No. 646

SCHEDULE 3 E+W+S SAMPLING AND TESTING METHODS

PART III E+W+S METHODS FOR THE ISOLATION OF SALMONELLA

Commencement Information

I1 Sch. 3 Pt. III in force at 1.4.1999, see art. 1

A. BACTERIOLOGICAL METHOD E+W+S

Day 3 E+W+S

4.  The RV broth shall be plated out on to two 90 millimetre plates of Brilliant Green Agar (BGA)(1) or on to one 90 millimetre plate of BGA and one 90 millimetre plate of Xylose Lysine Deoxycholate Agar (XLD)(2) using a 2.5 mm diameter loop. The plates shall be inoculated with a droplet taken from the edge of the surface of the fluid by drawing the loop over the whole of one plate in a zig zag pattern and continuing to the second plate without recharging the loop. The space between the loop streaks shall be 0.5 cm—1.0 cm. The plates shall be incubated at 37°C overnight.

(1)

Brilliant Green Agar—See Edel, W. and Kampelmacher, E. H. (1969) Bulletin of World Health Organisation, 41:297—306, World Health Organisation Distribution and Sales, CH-1211, Geneva 27, Switzerland (ISSN 0042—9686).

(2)

Xylose Lysine Deoxycholate Agar—See Taylor, W. I. (1965) American Journal of Clinical Pathology, 44:471—475, Lippincott and Raven, 227 E. Washington Street, Philadelphia PA19106, USA.