The Natural Mineral Water, Spring Water and Bottled Drinking Water (England) Regulations 2007

[F1PART 4E+W+SMethods of analyses relating to specified microbiological parameters

ParameterMethod of analysis
Escherichia coli (E. coli)BS EN ISO 9308-1:2014+A1:2017 entitled “Water quality — Enumeration of Escherichia coli and coliform bacteria. Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1:2014)” (ISBN 978 0 580 92379 1) published by the BSI on 31st October 2014, as amended on 28th February 2017, or BS EN ISO 9308-2:2014 entitled “Water quality — Enumeration of Escherichia coli and coliform bacteria. Part 2: Most probable number method” (ISBN 978 0 580 84023 4) published by the BSI on 30th June 2012, as amended by a corrigendum issued on 30th June 2014
EnterococciBS EN ISO 7899-2:2000, BS 6068-4.4:2000 entitled “Water quality — Detection and enumeration of intestinal enterococci — Part 2: Membrane filtration method” (ISBN 0 580 34953 5) published by the BSI on 15th August 2000
Pseudomonas aeruginosaBS EN ISO 16266:2008 entitled “Water quality — Detection and enumeration of Pseudomonas aeruginosa — Method by membrane filtration” (ISBN 978 0 580 59736 7) published by the BSI on 31st March 2008
Colony count 22ºC – enumeration of culturable microorganismsBS EN ISO 6222: 1999, BS 6068-4.5:1999 entitled “Water quality — Enumeration of cultural micro-organisms — Colony count by inoculation in a nutrient agar culture medium” (ISBN 0 580 32495 8) published by the BSI on 15th August 1999
Colony count 36ºC – enumeration of culturable microorganismsBS EN ISO 6222: 1999, BS 6068-4.5:1999 entitled “Water quality — Enumeration of cultural micro-organisms — Colony count by inoculation in a nutrient agar culture medium” (ISBN 0 580 32495 8) published by the BSI on 15th August 1999]