Regulation 3

SCHEDULE 2 Authorisation of a preparation of Lactobacillus buchneri (DSM 29026) (identification number 1k20759) as a feed additive for all animal species

The preparation of Lactobacillus buchneri (DSM 29026), belonging to the additive category ‘technological additives’ and to the functional group ‘silage additives’, is authorised as an additive in animal nutrition in accordance with the specifications in the following table.

Additive	Lactobacillus buchneri (DSM 29026)
(1) There is no requirement to include the name of the holder of this authorisation as this authorisation does not fall within the scope of Article 9(5) of Regulation (EC) 1831/2003.
(2) Details of the analytical methods set out in the document referenced “Ares(2019)4747322 – 22/07/2019” and last updated 18th October 2019. The document is available at the following address: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports/fad-2018-0093.
(3) BS EN 15787:2021 “Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Lactobacillus spp. used as feed additive”. Published by the British Standards Institution on 31st December 2021 (ISBN 978 0 580 99831 7). Available from the British Standards Institution https://knowledge.bsigroup.com.
(4) Content of Lactobacillus buchneri (DSM 29026) : CFU of additive/kg of fresh material.
(5) Easy to ensile forage: > 3 % soluble carbohydrates in fresh material; moderately difficult to ensile forage: 1.5-3.0 % soluble carbohydrates in the fresh material in accordance with Commission Regulation (EC) No.429/2008 on detailed rules for the implementation of Regulation (EC) No.1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of applications and the assessment and the authorisation of feed additives(1).
Identification number of the additive	1k20759
Authorisation holder(1)
Additive category	Technological additives
Functional group	Silage additives
Additive composition	Preparation of Lactobacillus buchneri (DSM 29026) containing a minimum of: 2 × 1010 CFU/g additive.
Characterisation of the active substance(s)	Viable cells of Lactobacillus buchneri (DSM 29026).
Analytical methods(2)	For enumeration (colony count) of the feed additive: Spread plate method on MRS agar in accordance with BS EN 15787:2021(3).
	For identification of bacterial strain: Pulsed Field Gel Electrophoresis (PFGE).
Species or category of animal	All animal species.
Maximum age	Not applicable.
Minimum content (4)	Minimum content of the additive when not combined with other micro-organisms as silage additives: 5x107 CFU/kg of easy and moderately difficult to ensile fresh material(5).
Maximum content(4)	No maximum.
Other provisions	In the directions for use of the additive and premixtures, the storage conditions must be indicated.

(1)

EUR 2008/429, amended by S.I. 2019/654. S.I. 2019/654 was itself amended by S.I.2020/1504.