Regulation 3

SCHEDULE 5Renewal of authorisation of a preparation of Saccharomyces cerevisiae (CNCM I-4407) (formerly Saccharomyces cerevisiae NCYC Sc.47) (identification number 4b1702) as a feed additive for calves for rearing

The preparation of Saccharomyces cerevisiae CNCM I-4407, belonging to the additive category ‘zootechnical additives’ and to the functional group ‘gut flora stabilisers’, is authorised as an additive in animal nutrition in accordance with the specifications in the following table(1).

(1)

Details of the analytical methods set out in the document referenced “JRC.DG.D.6/CvH/DM/mds/ARES(2010)967257” and last updated 6th June 2016. The document is available at the following address: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports/fad-2010-0038.

(2)

BS EN 15789:2021 “Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Saccharomyces cerevisiae used as feed additive”. Published by the British Standards Institution on 30th November 2021 (ISBN 978 0 580 99832 4). Available from the British Standards Institution https://knowledge.bsigroup.com.

(3)

DD CEN/TS:15790:2008 “Animal Feeding Stuffs – PCR typing of probiotic strains of Sacccharomyces cerevisiae (yeast)”. Published by the British Standards Institution on 31st January 2009 (ISBN 978 0 580 61806 2). Available from the British Standards Institution https://knowledge.bsigroup.com.

(4)

Content of Saccharomyces cerevisiae (CNCM I-4407): CFU/kg of complete feed with a moisture content of 12%.

AdditiveSaccharomyces cerevisiae (CNCM I-4407)
Identification number of the additive4b1702
Authorisation holderS.I. Lesaffre
Additive categoryZootechnical additives
Functional groupGut flora stabilisers
Additive compositionSolid form preparation of Saccharomyces cerevisiae (CNCM I-4407) containing a minimum of 5 × 109 CFU/g additive.
Characterisation of the active substance(s)Viable dried cells of Saccharomyces cerevisiae (CNCM I-4407).
Analytical methods(1)

For enumeration (colony count) in the feed additive, premixtures, feed materials and compound feed:

  • Pour plate method CGYE (chloramphenicol, glucose, yeast extract) agar in accordance with BS EN 15789:2021(2).

For identification of yeast strain:

  • Polymerase chain reaction (PCR) method in accordance with DD CEN/TS 15790:2008(3).

Species or category of animalCalves for rearing
Maximum ageNot applicable
Minimum content(4)1.5 x 10 9 CFU/kg
Maximum content(4)No maximum
Other provisionsIn the directions for use of the additive and premixtures, the storage conditions and stability to heat treatment must be indicated.
(1)

This authorisation is a renewal of the authorisation granted under Commission Regulation (EU) No. 883/2010. That Regulation is revoked by regulation 11 of these Regulations but see the transitional provision in regulation 9.