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Regulation 3(6)
Synonyms | |||
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Definition | Steviol glycosides produced via enzymatic conversion of highly purified steviol glycosides (rebaudioside A or stevioside) stevia leaf extracts are composed predominantly of rebaudioside M, rebaudioside D, and rebaudioside AM. Rebaudiosides D, M and AM are produced via enzymatic conversion of highly purified steviol glycoside (rebaudioside A or stevioside) extracts (95% steviol glycosides) obtained from Stevia rebaudiana Bertoni plant using UDP-glucosyltransferase and sucrose synthase enzymes produced by genetically modified strains of Escherichia coli (pPM294, pFAH170, and pSK041) that facilitate the transfer of glucose from sucrose and UDP-glucose to steviol glycosides via glycosidic bonds. After removal of the enzymes by solid-liquid separation and heat treatment, the purification involves concentration of the steviol glycosides by resin adsorption, followed by recrystallisation of the steviol glycosides resulting in a final product containing not less than 95 % of total steviol glycosides, including one or more of rebaudiosides D, M and AM. Viable cells or DNA of Escherichia coli (pPM294, pFAH170, and pSK041) must not be detected in the food additive. | ||
Chemical name | Rebaudioside M: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester Rebaudioside D: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester Rebaudioside AM: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester | ||
Molecular formula | Trivial name | Formula | Conversion factor |
Rebaudioside M | C56 H90O33 | 0.25 | |
Rebaudioside D | C50H80O28 | 0.29 | |
Rebaudioside AM | C50H80O28 | 0.29 | |
Molecular weight and CAS No | Trivial name | CAS Number | Molecular weight (g/mol) |
Rebaudioside M | 1220616-44-3 | 1291.29 | |
Rebaudioside D | 63279-13-0 | 1129.15 | |
Rebaudioside AM | 2222580-26-7 | 1129.15 | |
Assay | Not less than 95 % of steviol glycosides on the dried basis, including one or more of rebaudiosides D, M and AM. | ||
Description | White to light yellow powder, approximately between 200 and 350 times sweeter than sucrose (at 5 % sucrose equivalency). | ||
Identification | |||
Solubility | Freely soluble to slightly soluble in water. | ||
pH | Between 4.5 and 7.0 (1 in 100 solution) | ||
Purity | |||
Total ash | Not more than 1 % | ||
Loss on drying | Not more than 6 % (105 °C, 2h) | ||
Residual solvent | Not more than 5000 mg/kg ethanol | ||
Arsenic | Not more than 0.015 mg/kg | ||
Lead | Not more than 0.2 mg/kg | ||
Cadmium | Not more than 0.015 mg/kg | ||
Mercury | Not more than 0.07 mg/kg | ||
Residual protein | Not more than 5 mg/kg” |
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