PART 2Analytical methods
Table 1
Prescribed methods of analysis
| Parameter | Method | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
* Use the following method to make m-CP agar : Make a basal medium consisting of—
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool. Dissolve—
into 8ml sterile water and add it to the medium. Add to the medium—
| |||||||||||||||||||||||||||
| Clostridium perfringens (including spores) | Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds. | ||||||||||||||||||||||||||
| Coliform bacteria | BS-EN ISO 9308-1 | ||||||||||||||||||||||||||
| Colony count 22°C — enumeration of culturable microorganisms | BS-EN ISO 6222 | ||||||||||||||||||||||||||
| Colony count 37°C — enumeration of culturable microorganisms | BS-EN ISO 6222 | ||||||||||||||||||||||||||
| Enterococci BS-EN ISO 7899-2 | |||||||||||||||||||||||||||
| Escherichia coli (E. coli) | BS-EN ISO 9308-1 | ||||||||||||||||||||||||||
| Pseudomonas aeruginosa | BS-EN-ISO 12780 | ||||||||||||||||||||||||||
Table 2
Prescribed performance characteristics for methods of analysis
| Parameters | Trueness % of prescribed concentration or value or specification | Precision % of prescribed concentration or value or specification | Limit of detection % of prescribed concentration or value or specification |
|---|---|---|---|
| Notes: | |||
(i) The method of analysis should determine total cyanide in all forms. | |||
(ii) The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned. | |||
(iii) The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1. | |||
(iv) The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1. | |||
(v) The performance characteristics apply to the prescribed value of 4 NTU. | |||
(vi) The performance characteristics apply to the specification of 1 NTU for surface waters or ground waters influenced by surface water. | |||
| Aluminium | 10 | 10 | 10 |
| Ammonium | 10 | 10 | 10 |
| Antimony | 25 | 25 | 25 |
| Arsenic | 10 | 10 | 10 |
| Benzene | 25 | 25 | 25 |
| Benzo(a)pyrene | 25 | 25 | 25 |
| Boron | 10 | 10 | 10 |
| Bromate | 25 | 25 | 25 |
| Cadmium | 10 | 10 | 10 |
| Chloride | 10 | 10 | 10 |
| Chromium | 10 | 10 | 10 |
| Colour | 10 | 10 | 10 |
| Conductivity | 10 | 10 | 10 |
| Copper | 10 | 10 | 10 |
| Cyanide (i) | 10 | 10 | 10 |
| 1,2-dichloroethane | 25 | 25 | 10 |
| Fluoride | 10 | 10 | 10 |
| Iron | 10 | 10 | 10 |
| Lead | 10 | 10 | 10 |
| Manganese | 10 | 10 | 10 |
| Mercury | 20 | 10 | 20 |
| Nickel | 10 | 10 | 10 |
| Nitrate | 10 | 10 | 10 |
| Nitrite | 10 | 10 | 10 |
| Pesticides and related products (ii) | 25 | 25 | 25 |
| Polycyclic aromatic hydrocarbons (iii) | 25 | 25 | 25 |
| Selenium | 10 | 10 | 10 |
| Sodium | 10 | 10 | 10 |
| Sulphate | 10 | 10 | 10 |
| Tetrachloroethene (iv) | 25 | 25 | 10 |
| Tetrachloromethane | 20 | 20 | 20 |
| Trichloroethene (iv) | 25 | 25 | 10 |
| Trihalomethanes: | |||
| Total (iii) | 25 | 25 | 10 |
| Turbidity (v) | 10 | 10 | 10 |
| Turbidity (vi) | 25 | 25 | 25 |