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The Water Supply (Water Quality) Regulations 2010 (revoked)

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Version Superseded: 15/06/2018

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Point in time view as at 31/03/2017.

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There are currently no known outstanding effects for the The Water Supply (Water Quality) Regulations 2010 (revoked), Table A1 . Help about Changes to Legislation

Regulation 16

Table A1 E+WParameters for which, Subject to Regulation 16(7), Methods of Analysis are Prescribed

(1)(2)
ParameterMethod
Clostridium perfringens (including spores)Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
Coliform bacteriaISO 9308-1
Colony count 22°C-enumeration of culturable microorganismsprEN ISO 6222
Colony count 37°C-enumeration of culturable microorganismsprEN ISO 6222
EnterococciISO 7899-2
Escherichia coli (E coli)ISO 9308-1
*The composition of m-CP agar is:
Basal medium
Tryptose30.0g
Yeast extract20.0g
Sucrose5.0g
L-cysteine hydrochloride1.0g
MgSO4·7H2O0.1g
Bromocresol purple40.0mg
Agar15.0g
Water1,000.0ml
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
D-cycloserine400.0mg
Polymyxine-B sulphate25.0mg
Indoxyl-[beta]-D-glucoside to be dissolved in 8 ml sterile water before addition60.0mg
Filter-sterilised 0.5% phenolphthalein diphosphate solution20.0 ml
Filter-sterilised 4.5% FeCl3·6H2O2.0 ml

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