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This is the original version (as it was originally made).
Regulation 16
Table A1Parameters for which, Subject to Regulation 16(7), Methods of Analysis are Prescribed
(1) | (2) |
---|
Parameter | Method |
---|
Clostridium perfringens (including spores) | Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds. |
Coliform bacteria | ISO 9308-1 |
Colony count 22°C-enumeration of culturable microorganisms | prEN ISO 6222 |
Colony count 37°C-enumeration of culturable microorganisms | prEN ISO 6222 |
Enterococci | ISO 7899-2 |
Escherichia coli (E coli) | ISO 9308-1 |
*The composition of m-CP agar is: | |
Basal medium | |
Tryptose | 30.0g |
Yeast extract | 20.0g |
Sucrose | 5.0g |
L-cysteine hydrochloride | 1.0g |
MgSO4·7H2O | 0.1g |
Bromocresol purple | 40.0mg |
Agar | 15.0g |
Water | 1,000.0ml |
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add: | |
D-cycloserine | 400.0mg |
Polymyxine-B sulphate | 25.0mg |
Indoxyl-[beta]-D-glucoside to be dissolved in 8 ml sterile water before addition | 60.0mg |
Filter-sterilised 0.5% phenolphthalein diphosphate solution | 20.0 ml |
Filter-sterilised 4.5% FeCl3·6H2O | 2.0 ml |
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