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The Water Supply (Water Quality) Regulations 2010

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This is the original version (as it was originally made).

Regulation 16

Table A1Parameters for which, Subject to Regulation 16(7), Methods of Analysis are Prescribed

(1)(2)
ParameterMethod
Clostridium perfringens (including spores)Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
Coliform bacteriaISO 9308-1
Colony count 22°C-enumeration of culturable microorganismsprEN ISO 6222
Colony count 37°C-enumeration of culturable microorganismsprEN ISO 6222
EnterococciISO 7899-2
Escherichia coli (E coli)ISO 9308-1
*The composition of m-CP agar is:
Basal medium
Tryptose30.0g
Yeast extract20.0g
Sucrose5.0g
L-cysteine hydrochloride1.0g
MgSO4·7H2O0.1g
Bromocresol purple40.0mg
Agar15.0g
Water1,000.0ml
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
D-cycloserine400.0mg
Polymyxine-B sulphate25.0mg
Indoxyl-[beta]-D-glucoside to be dissolved in 8 ml sterile water before addition60.0mg
Filter-sterilised 0.5% phenolphthalein diphosphate solution20.0 ml
Filter-sterilised 4.5% FeCl3·6H2O2.0 ml

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