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The Feed Additives (Authorisations) (Wales) Regulations 2022
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Regulation 3(1)
SCHEDULE 3Authorisation of a preparation of serine protease (EC 3.4.21.-) produced by Bacillus licheniformis DSM 19670 (identification number 4a13) as a feed additive for chickens for fattening
The preparation specified in the table, belonging to the additive category “zootechnical additives” and to the functional group “digestibility enhancers”, is authorised as an additive in animal nutrition subject to the conditions set out in the table.
| (1) One PROT is the amount of serine protease that liberates one micromole/minute of para-nitroaniline (pNA) from 1 millimolar (mM) Suc-Ala-Ala-Pro-Phe-pNA substrate at pH 9 & 37°C. | |
| (2) CAS Registry Number® assigned to this preparation by the Chemical Abstracts Service https://www.cas.org/cas-data/cas-registry. | |
| (3) European Inventory of Existing Commercial Substances, number as published in OJ No C 146 A, 15.6.90, p.1. | |
| (4) Identification number assigned by the International Union of Biochemistry (IUB), now the International Union of Biochemistry and Molecular Biology (IUBMB) iubmb.org. | |
| (5) Details of the analytical methods set out in the document referenced “Ares(2019)6802984 - 04/11/2019” and last updated 27 January 2020. The document is available at the following address: https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-reports/fad-2019-0010. | |
| (6) Content of Serine protease in PROT/kg of complete feed with a moisture content of 12%. | |
| Additive | Serine protease (EC 3.4.21.-) |
| Identification number of the additive | 4a13 |
| Authorisation holder | DSM Nutritional Products Ltd (Switzerland) |
| Additive category | Zootechnical additives |
| Functional group | Digestibility enhancers |
| Additive composition | Solid and liquid preparation of serine protease (EC 3.4.21.-):
|
| Characterisation of the active substance(s) | |
| Analytical methods (5) | For quantification of serine protease activity in the feed additive, premixtures, compound feed and feed materials:
|
| Species or category of animal | Chickens for fattening |
| Maximum age | None |
| Minimum content(6) | 15,000 PROT(1)/kg |
| Maximum content(6) | None |
| Other provisions | In the directions for use of the feed additive and premixtures, the storage conditions and stability to heat treatment must be indicated. |
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